Immunohistochemical Study Of Perineural Invasion Growth Mechanism In Lacrimal Gland Adenoid Cystic Carcinoma

ObjectiveTo detect the expression and correlation of S100, GFAP, Leu-7in adenoid cystic carcinoma of lacrimal gland with different histological types as well as their relations with perineural invasion (PNI) of adenoid cystic carcinoma(ACC) of lacrimal gland by immunohistochemical staining. It is further provide experimental evidence for the mechanisms of PNI in ACC of lacrimal gland. We would grasp their interaction and association in the microenvironment of ACC to provide references for clinically effective.Methods(1) Collect the tissue sections of30patients as adenoid cystic carcinoma of lacrimal gland and5normal lacrimal gland.(2) Collect the clinical and pathological information of these patients.(3) Immunohistochemical staining.(4) Detect the expression of S100, GFAP, Leu-7in adenoid cystic carcinoma of lacrimal gland as well as their expression and nerve involvement rates in different groups.(5) The data was analyzed by SPSS19.0statistical software. P<0.05was regarded as the standard of statistical significance.Results1. The pathological diagnosis of the tissue sections of30adenoid cystic carcinoma of lacrimal gland is consistant with the original tissue.2. S100and GFAP expressed in tissue sections of adenoid cystic carcinoma of lacrimal gland in different levels. Leu-7doesn’t expressed in the tumor tissue sections and normal lacrimal gland. S100mainly expressed in the cytoplasm and nucleus. The S100expression rate is86.7%. The GFAP expression rate is83.3%that expressed in the cytoplasm in the tumor tissue sections. Their expression rates were significantly higher than normal tissue. Poorly differentiated/recurrence ACC PNI rate was significantly higher than that of well-differentiated/primary group PNI rate, the difference was statistically significant (P<0.05). 3. S100and GFAP expression rates of poorly differentiated ACC organizations were significantly higher than the rate of their expression in well-differentiated organizations; S100and GFAP expression rates of perineural invasion group ACC organizations were significantly higher than the rates of their expression in no perineural invasion group ACC organizations, the difference were statistically significant (P<0.05).Conclusion1. Adenoid cystic carcinoma of lacrimal gland has the neurotropic invasive biological behavior, one of the indicators of perineural invasion phenomenon as a lacrimal gland ACC clinical observation of poor prognosis.2. The poorly differentiation of the lacrimal gland ACC has higher nerve involvement rates; recurrent lacrimal gland ACC is easier invading the nervous compared with primary lacrimal gland ACC, it may be clinically cause varying degrees neurological symptoms such as pain and numbness.3. S100, GFAP respectively expressed higher positive rate in the neurotropic group, recurrent group, poorly differentiated group. It can be speculated that the S100,GFAP plays a certain role in the adenoid cystic carcinoma of lacrimal gland perineural invasion, both as the phenomenon of biological indicators to judge neurotropic invasion.4. Leu-7expression was negative in the lacrimal gland ACC and normal lacrimal gland tissue, suggesting no clear correlation with the nature of the lesion and the neural invasion of the adenoid cystic carcinoma of lacrimal gland.5. The neurotropic mechanism about Schwann cell differentiation in adenoid cystic carcinoma of lacrimal gland, still need a large sample and a variety of markers of experiments confirmed, the sensitivity and specificity of the different Schwann cell markers are different, seeking PNI-specific indicators of the adenoid cystic carcinoma of lacrimal gland will be an important goal.