Protective Effects Of Hydrogen-rich Lactated Ringer’s Solution On Liver Cold Ischemia/reperfusion Injury In Rats

Objective:Recently it has been demonstrated that hydrogen can selectively reduce hydroxyl radicals (·OH)and peroxynitrite (ONOO-), thus protect against oxidative damage. This study was designed to investigate the effects of hydrogen-rich lactated Ringer’s solution on liver cold ischemia/reperfusion (I/R) injury in rats.Methods: Twenty-four healthy male Sprague-Dawley rats were divided randomly into three experimental groups (n=8each):sham operation group (group S), liver cold I/R plus lactated Ringer’s solution treatment group (group RL) and liver cold I/R plus hydrogen-rich lactated Ringer’s solution treatment group (group HRL). Group S only underwent simple laparotomy. In group RL and HRL, the liver was perfused with lactated Ringer’s solution and hydrogen-rich lactated Ringer’s solution respectively. The liver cold ischemia time was20min, and6h after reperfusion, blood samples were obtained to detect the levels of serum alanine amino-transferase (ALT), aspartate amino-transferase (AST), TNF-α and IL-6. Hepatic specimens were obtained to evaluate the pathological changes, and also to detect the levels of malondialdehyde (MDA) and superoxide dismutase (SOD). The expression of activated caspase-3was detected by the immunochemistry, the apoptosis of liver was measured by TUNEL assay, the expression of NF-κB mRNA was analyzed by real-time PCR. Statistical analysis was carried out with SPSS17.0.Results:As compared with group RL, hydrogen-rich lactated Ringer’s solution significantly alleviated liver damage. As compared with group S, the levels of ALT, AST, TNF-α, IL-6and MDA, the expression of activated caspase-3and NF-κB mRNA, and the percentage of TUNEL-positive cells were significantly increased in groups RL and HRL, while the level of SOD was decreased (P<0.05or0.01). As compared with group RL, the levels of ALT, AST, TNF-α, IL-6and MDA, the expression of activated caspase-3and NF-κB mRNA, and the percentage of TUNEL-positive cells were significantly decreased in group HRL, while the level of SOD was increased (P<0.05).Conclusion:Hydrogen-rich lactated Ringer’s solution can obviously attenuate liver cold I/R injury in rats, with the characteristic of lower serum levels of ALT and AST, and less apoptosis cells. The possible mechanisms may be hydrogen can selectively reducing cytotoxic oxygen radicals, play an anti-oxidant and anti-inflammatory role in liver ischemia reperfusion injury.