Effects Of The Inzone Preload On The Glucose And Lipid Metabolism, Body Weight In Type2Diabetes

Objectives:To observe the effects of the Inzone preload on the glucolipid metabolism and body weight in type2diabetes patients with postprandial blood glucose poorly controled for3months.Methods:Through a before-after study, thirty T2DM subjects, including19men and11women with mean age of54.8±7.8years, who took two or three kinds of hypoglycemic agents but still had poorly controlled postprandial blood glucose were enrolled in a12-week program. The subjects were not permission to change the life-style and drug therapy during the study. All subjects were administered with the Inzone30min before each meal during the study period. Fasting glucose, postprandial2h glucose and anthropometrical parameters were monitored each3weeks during the whole study. Fasting plasma glucose, glycosylated hemoglobin, serum lipids, fasting insulin, C-reactive protein and homeostasis model assessment were evaluated before and after intervention. The appetite of the subjects were assessed using visual analogue scales after the preload.Results:1. Compared with baseline values, fasting blood glucose were reduced0.2±0.1mmol/L, but it was no significance (p>0.05); Reductions in2h-BG were found near maximal at week4, with modest progressive reductions and no apparent plateau observed through12weeks periods. Overall2h-BG and HbAlc values were significantly reduced with an average change of1.7±1.3mmol/L and0.3±0.2%, respectively (p<0.05)2. Compared with baseline values, overall serum total cholesterol and LDL concentrations were significantly reduced by0.5±0.2mmol/1and0.3±0.1mmol/1respectively (p<0.05). Mean baseline serum triglycerides rose from1.7±0.6to1.8±0.8mg/dL, but the differences did not reach significant levels (p>0.05. In addition, no significant changes were observed in HDL-c during the intervention period (p>0.05).3. At the end of the12-week intervention period, the mean reductions in body weight was0.9±0.5kg, waist circumference and hip circumference were decreased0.9±0.7and0.1±0.1cm respectively, but the changes were not significantly different (p>0.05).4. Compared with baseline values, the mean change in HOMA-IR and HOMA-β was-0.8±0.5and7.8±5.2, however it was no statistical significance (p>0.05).5. The Inzone preload could obviously increase the satiety (p<0.05).6.There were no significant changes in the blood routine examination, serum nitrogen and creatinine, hepatic transaminases,compared to those at the baseline (p>0.05), Compared with baselin, mean C-reactive protein significantly reduced0.7±0.6mg/L (p<0.05).7. The Inzone preload were well adopted and no adverse effects were observed during the study.Conclusion The inzone preload intervetion could significantly reduce postprandial blood glucose and HbAlc cholesterol and low density lipoprotein cholesterol,C-reactive protein and increase satiety in patients with type2diabetes. For some patients, this kind of dietary interventions have a favorable effect on weight and waist circumference. Inzone preload could be as a dietary strategy in the diabetes management. Part Ⅱ Effects of the inzone preload on glucose metabolism, insulin and GLP-1in streptozotocin induced diabetic rats.AbstractObjective: To observe the effects of the inzone preload on glucose metabolism, body weight in streptozotocin induced diabetic rats. Meanwhile, we observed the impacts of the inzone preload on blood glucose, serum levels of insulin, plasma Glucagon like peptide1(GLP-1), combining GLP-1in ileum, to explore the influence of the inzone preload on glucose metabolism and and the mechanism to reduce postprandial blood glucose.Methods: Streptozotocin induced diabetic rats were established with the method of low-dose STZ (30mg/kg) injection from tail vein after feeding male Sprague-Dawley rats with four weeks’ high-sucrose-fat diet. The DM model rats were randomly divided into control group (DC group, n=6),and inzone treated group(DT group, n=6) fed with regular high-sucrose-fat diet mixed with inzone for12weeks,respectively. Body weight and random blood glucose (RBG) were monitored during the feeding period. The oral glucose tolerance test (OGTT) was administered to diabetic rats at the end of the experiment. Glucose (2g/kg) was given orally, and blood glucose, insulin and GLP-1levels were measured at-30,0,10,20,30,60, and120min after glucose loading, the inzone were administrated orally30min before OGTT in intervetion group. Glucagon like peptide1(GLP-1) were measured after executing the rats. The expression of GLP-1in ileum were studied by immunohistochemical method.Results:1.Body weight: Compared with control group, the body weight in DC group was decreased, but there were no significant differences (p>0.05).At8weeks, the weight of was significantly lower than control group since the4th week and maintained to12weeks.2.RBG:There was an decreased tend of blood glucose in the DC group, but there were no significant differences (p>0.05). Compared with DC group, there were no significant changes in random blood sugar at0,4,8and12weeks between two groups.3.OGTT: Compared with non-inzone preload, the area under the curve(AUC) of glucose was lower after the inzone preload. The serum insulin levels in0-20min and the GLP-1in0-60min were significantly higher after the inzone preload. The insulin was also significantly increased at0-20min, then reached peak at30min, but the peak value was slightly higher than in inzone preload. The area under the curve(AUC) of insulin and GLP-1was higher after the inzone preload (p<0.05). There were no significant changes between the two group (p>0.05).4.GLP-1secretion in ileum:Compared with non-inzone preload, the GLP-1secretion in ileum levels were significantly higher after the inzone preload (p<0.05). But there were no significant changes between two group (p>0.05).5.GLP-1expression: Compared with non-inzone preload, the Integral Optical Density of GLP-1increased in DC group and DT group after the inzone preload intervention (p<0.05).But there were no significant changes between two group (p>0.05).Conclusions:1.The inzone supplementary diet resulted in body weight loss but did not change the random blood glucose.2.The postprandial blood glucose was significantly lower after the inzone preloads, The iAUCs for insulin, GLP-1were greater when the inzone was given as a preload compared with no preload, indicating that the inzone preload can stimulate insulin and GLP-1secretion, leading to marked reduction in postprandial glycemia. The inzone preload might be a promising nutritional strategies in the management of diabetes.