| Objective:Transforming acidic coiled-coil proteins3is one of the important members of the TACC protein family, this protein family are thought to be associated with the cytoskeleton in the regulation of microtubule movement, associated with the development of tumor. TACC3interaction with the p53, if the level changes TACC3, spindle cell function may be affected, when the p53-dependent checkpoint which would not be detected by mitosis, and then the next cell cycle pathway triggers apoptosis, if the lack of p53gene in the cells, cells can’t be normal mitosis, and changes in the level of TACC3are not sufficient to induce cell apoptosis, leading to genetic instability, and induce the occurrence of tumor. This report is to investigate the expression of TACC3and p53in bladder transitional cell carcinoma and normal mucosa by immunehistochemistry, analyze the clinical pathological features.Methods:This study included84cases.According to the WHO2004classification method and the international federation of cancer (UICC)2009version7TMN staging method of grading and staging of bladder transitional cell carcinoma. The research obtained the approval of the Second Hospital of Tianjin Medical University Medical Ethics Committee.1.The expression of TACC3and p53in76cases of bladder transitional cell carcinoma and8cases of normal mucous was detected by immunohistochemistry, and the clinical pathological features were analyzed.2. Statistical:Ali datas were analyzed by SPSS17.0statistical software package. Statistical significance was set at P<0.05.Results:1.TACC3is mainly located in cytoplasm, increased as pathological staging, accompanied by staining nucleus.Normal bladder mucosa epithelial not express or weakly positive expression.In76cases of bladder transitional cell carcinoma positive and strong positive expression of a total of48cases, higher expression rate was63.18%(48/76). while in normal bladder mucosa epithelium is0(0/18).TACC3high expression and patients age, sex and tumor size differences had no statistical significance;And with tumor differentiation degree and pathological staging and smoking between difference was statistically significant (P<0.05).2.p53mainly located in nucleus,normal bladder mucosa did not express.In76cases of bladder transitional cell carcinoma positive and strong positive expression, a total of44cases, higher expression rate was57.89%(48/76), while in normal bladder mucosa epithelium is0(0/8).The high expression of p53and patients’age, sex, tumor size and the difference between smoking without statistical significance;and with tumor differentiation degree and the difference between the pathological staging was statistically significant (P<0.05).3.In bladder transitional cell carcinoma TACC3expression intensity was positively related to the intensity of the expression of p53(r=0.454, P<0.01).Conclusions:1. The expression strength and high expression rate of TACC3and p53in bladder transitional cell carcinoma were significantly higher than that in normal mucosa with statistically difference, the over-expression of TACC3and p53were relevant with the development and progression of bladder transitional cell carcinoma.2. The expression strength and high expression rate of TACC3and p53were positively related with tumor differentiation degree and pathological stage, they may play an important role in invasive of bladder transitional cell carcinoma.3. The expression strength and high expression rate of TACC3were positively related with smoking, and have no correlation between the expression of p53with smoking, but still need to enlarge the sample size to be further studying.4. Positive correlation was observed in bladder transitional cell carcinoma between TACC3and p53, it suggested that they may play a synergetic effect in the development, progression and metastasis of bladder transitional cell carcinoma. |
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