Study On The Relationships Between Syndrome Types Of TCM And T Cell Subsets, Immunoglobulin And Complement Levels In HBV Infection Chronic Liver Disease

Objective: We aims to explorer the immune function in different syndrome types ofpatients with HBV infection chronic liver disease, which was performed by observingthe distribution of syndrome types of Traditional Chinese Medicine (TCM) and thechange of T cell subsets, immunoglobulin and complement levels of the patients in thestages of chronic hepatitis, cirrhosis and liver cancer, at last, we expected to provide anew method for clinical diagnosis and treatment.Methods: We included patients that met criteria, and divided them into differentsyndrome types, their T cell subsets, immunoglobulin and complement level weredetected, and the differences of index level between different syndrome types werealso analyzed subsequently.Results:①We included a total of266cases. There are109cases with chronichepatitis B, their distribution of syndrome types of TCM according to the frequencywere: liver stagnation and spleen deficiency syndrome> liver depression and qistagnation syndrome> syndrome of accumulated dampness-heat> syndrome of liverand kidney yin deficiency> syndrome of spleen and kidney yang deficiency. Thereare87cases with cirrhosis, their distribution of syndrome types of TCM according tothe frequency were: syndrome of liver-qi stagnation> syndrome of accumulateddampness-heat> syndrome of water-dampness stagnation> syndrome of liver andkidney yin deficiency. There are70cases with liver cancer, their distribution ofsyndrome types of TCM according to the frequency were: liver depression and qistagnation syndrome> syndrome of stagnant dampness-heat> syndrome of spleendeficiency and dampness stagnation> yin deficiency syndrome> blood stasissyndrome.②There were no significant differences in T cell subsets level amongdifferent syndrome types of patients with chronic hepatitis B (P>0.05). The comparison of immunoglobulin and complement level among different syndrometypes were: the IgA level of liver depression and qi stagnation syndrome was lowerthan syndrome of accumulated dampness-heat and liver stagnation and spleendeficiency syndrome (P <0.05), which indicated that there were statisticallysignificant differences between them.③The comparison of T cell subsets level amongdifferent syndrome types of patients with liver cirrhosis were: the CD3+level ofsyndrome of liver-qi stagnation was higher than syndrome of accumulateddampness-heat (P <0.05), and the CD3+CD4+level was higher than syndrome ofaccumulated dampness-heat and syndrome of water-dampness stagnation (P <0.05),which indicated that there were statistically significant differences between them. Thecomparison of immunoglobulin and complement level among different syndrometypes were: the IgG level of syndrome of liver-qi stagnation was lower than syndromeof accumulated dampness-heat and syndrome of water-dampness stagnation andsyndrome of liver and kidney yin deficiency (P <0.05), the IgE level was lower thansyndrome of accumulated dampness-heat (P <0.05), the C3level was higher thansyndrome of accumulated dampness-heat (P <0.05), which indicated that there werestatistically significant differences between them.④There were no significantdifferences in T cell subsets level among different syndrome types of patients withliver cancer (P>0.05). The comparison of immunoglobulin and complement levelamong different syndrome types were: the IgA level of liver depression and qistagnation syndrome was lower than syndrome of stagnant dampness-heat and yindeficiency syndrome (P <0.05), the IgM level was lower than syndrome of stagnantdampness-heat (P <0.05), the C3level was higher than syndrome of stagnantdampness-heat and yin deficiency syndrome (P <0.05), which indicated that therewere statistically significant differences between them.⑤The comparison of T cellsubsets among different stages of patients with HBV infection chronic liver diseasewere: the CD3+,CD3+CD4+and CD3+CD8+levels of chronic hepatitis group werehigher than liver cirrhosis group and liver cancer group (P <0.05), the CD4+/CD8+ratio was lower than liver cirrhosis group and liver cancer group (P <0.05), theCD3+CD8+%level was higher than liver cirrhosis group (P <0.05), the CD3+%levelwas higher than liver cancer group (P <0.05), which indicated that there werestatistically significant differences between them. The comparison of immunoglobulinand complement level among different stages were: the IgG, IgA and IgM level ofchronic hepatitis group were lower than liver cirrhosis group (P <0.05), the IgG, IgA level were lower than liver cancer group (P <0.05), which indicated that there werestatistically significant differences between them.Conclusion:①The main syndromes of TCM in patients with HBV infection chronicliver disease were liver depression, qi stagnation, spleen deficiency anddampness-heat.②The immune function of patients with HBV infection chronic liverdisease were disordered, there were certain differences in T cell subsets,immunoglobulin and complement levels between different syndrome types andregularities in change of index level between different clinical stages.