| Objective In recent years, the incidence of cancer rises year by year, a serious threatto human health and life, and is currently one of the important means of treatment oftumor chemotherapy, but due to the lack of specificity of the chemotherapy drugchoice function, at the same time of killing tumor cells also kill the normal cells of thehuman body some normal function or destroy the human body. Clinical tumorpatients after chemotherapy can directly cause damage to the part of patients withliver function, then can happen liver cell necrosis, bile YuJi, cirrhosis of the liverpathological changes, etc. This not only influence the patient’s quality of life, andeven lead to patients give up chemotherapy and missed opportunity. A large numberof scientific research results indicate that acupuncture and moxibustion in thetreatment of chemotherapy in drug-induced liver injury has good curative effect, butabout acupuncture treatment after chemotherapy tumor-burdened bone protein contentin mice liver tissue has yet to do further study of the influence of abnormal.This subject adopts the S180tumor-burdened mice, one-time intraperitonealinjection of cyclophosphamide (CXT), drug-induced liver injury caused bychemotherapy in mice model, through the liver tissue of mice after chemotherapy ofreduced glutathione (GSH) and malondialdehyde (MDA) expression level of research,in order to investigate acupuncture to improve after chemotherapy tumor-burdenedmechanism of liver injury in mice.Methods Choose healthy7weeks, weight22, plus or minus2g male kunming miceto establish S180sarcoma transplantation model, according to the weight afterweighing a tumor-burdened mice were randomly divided into blank group, model group, acupuncture group, moxibustion group4. To CTX according to the dose of150mg/kg through abdominal cavity injected into model group, acupuncture group andmoxibustion group of S180mice tumor-burdened, blank group was injected into thecorresponding dose of0.9%saline solution. According to the pharmacokinetic model,namely after4h after the success of the building, acupuncture and moxibustiongroups respectively take "Da zhui","ge shu","shen shu","zu san li" for acupunctureand moxibustion therapy. Once a day for5days treatment. At the same time everyday to cut tail in mice blood, test number of peripheral white blood cells in mice.Treatment for five days to death mice, take1/4of section size in liver hepatic lobule,immediately put in4℃saline fixed2hours, standby ELISA test. Enzyme linkedimmunosorbent (ELISA) to observe the liver tissue of reduced glutathione (GSH) andmalondialdehyde (MDA) in protein positive expression. Data analysis usingSPSS19.0statistical analysis software for Windows.Results1. The building before and after the building group a tumor-burdened mice comparingwhite blood cell count, blank group before and after building no difference (P>0.05),model group, acupuncture group, moxibustion group before and after the building hasa significant difference (P <0.05), suggesting CTX tumor-burdened copy thesuccessful result of low white blood cells in mice model.2. Treatment for five days, the acupuncture group, moxibustion group atumor-burdened rising number of peripheral white blood cells in mice, theacupuncture group is higher than the blank group, model group, there were significantdifferences (P <0.05). Acupuncture and moxibustion group compared with nosignificant difference (P>0.05). But acupuncture group is higher than themoxibustion group.3. The expression of GSH. Compared with the blank group, model group atumor-burdened GSH content in liver tissue of mice significantly reduced (P <0.001);Compared with model group, acupuncture group and moxibustion group atumor-burdened increased content of GSH in liver tissue in mice (P <0.001). Compared to acupuncture and moxibustion group, acupuncture group increasedsignificantly in the moxibustion group, but the two groups have no significantdifference (P>0.05).4. The expression of MDA. Compared with the blank group, model group atumor-burdened MDA content in liver tissue of mice increased significantly (P<0.001); Compared with model group, acupuncture group and moxibustion group atumor-burdened MDA content in mice liver tissue reduce (P <0.05); Acupuncturegroup and moxibustion group, compared the moxibustion acupuncture group weresignificantly lower, but the two groups have no significant difference (P>0.05).Conclusions1. Groups before building a tumor-burdened mice peripheral blood leukocyte countthe difference is not obvious, the statistical difference between groups, suggests that abalanced distribution of white blood cell count, each a tumor-burdened mice can beused in the experiment. After building in addition to the normal group, other groups ofleukocyte tumor-burdened mice were decreased, compared before and after thebuilding has a significant difference, suggests cyclophosphamide chemotherapy drugsinhibit bone marrow, destroy the hematopoietic microenvironment, killing the sideeffects of white blood cells is very big, CTX tumor-burdened chemotherapy miceleukopenia model copy success.2. Acupuncture can promote peripheral blood leukocytes. Acupuncture reduce bonemarrow suppression after chemotherapy, improve the hematopoieticmicroenvironment, elevated white blood cell of curative effect is accurate, the effectwith treatment is enhanced by the increase in the number of times, and theacupuncture group recovered quickly, consistent with the previous tutor group results.3. Acupuncture and moxibustion can increase a tumor-burdened expression level ofGSH in liver tissue in mice, help clean up oxygen free radical formed bychemotherapy drugs combined into low toxic substance or its metabolites, makes cellsmore easily into a state of preservation and restoration of liver function, inhibition oflipid peroxidation, reducing free radicals attack of DNA, thus reduce DNA damage and mutation. Experimental results showed that acupuncture can increase theexpression of a tumor-burdened GSH in liver tissue in mice, and the curative effect ofacupuncture good something moxibustion.4. Acupuncture and moxibustion can cut a tumor-burdened expression level of MDAin liver tissue in mice, to regulate the production of the liver immune cells, the proteincontent, lower lipid peroxidation of unsaturated fatty acid in the process of oxidationdecomposition releases the reactivity of aldehydes, enhance the function of immunecells in the liver, improve the ability of antioxidant defense system, inhibition of lipidperoxidation, and exerted a great of liver damage after chemotherapy has a role toimprove effect and decrease toxicity. The experimental results showed thatacupuncture can reduce a tumor-burdened the expression of MDA in liver tissue inmice, and the curative effect of acupuncture good something moxibustion.In a word, the research results indicate that acupuncture and moxibustion can improvebone marrow hematopoietic microenvironment after chemotherapy, increaseperipheral blood leukocytes. At the same time, through the acupuncture andmoxibustion increase a tumor-burdened expression level of GSH in liver tissue ofmice and cut a tumor-burdened expression level of MDA in liver tissue in mice,regulate the production of liver cells, protein content, and let the cells more easily intoa state of preservation and restoration of liver function, enhance the function ofimmune cells in the liver, improve the ability of antioxidant defense system, thus toliver damage after chemotherapy has a role to improve effect and decrease toxicity,acupuncture is to benign tumor after chemotherapy the body liver function one of theways to adjust action. |
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