Experimental Research On Cryopreservation Allograft Vascular Transplantation Of Rabbit

Objects：This experiment used rabbit femoral artery,which were saved different timeunder4℃low temperature preservation condition, for allograft vascular transplantation,then observe the general morphology of the grafts after transplantation, vascular patencyrate, anastomotic thrombosis, histological changes and the change of surroundinginflammatory response, and comparing with fresh autogenous grafts, explore feasibilityof allograft vascular transplantation, and so as to find out in the cryopreservation cases,the best time of allogeneic artery in vitro preservation, and to establish simple vasculartransplantation bank.Methods：Select6months healthy Japanese big ear rabbit,2-2.5kg weight, male andfemale unlimited, surgery for bilateral femoral artery save in organ transplantationperfusate,4℃low temperature preservation different times, and standby for vasculargrafts. Experimental animals were randomly divided into four groups: A fresh bloodvessels of autologous vascular transplantation; B fresh allograft vascular transplantation;C allograft vascular transplantation after keeped24hours; D allograft vasculartransplantation after savced48hours. There are24rabbits in each group. Each animalwas performed ordinary heparin anticoagulation for two weeks after the completion oftransplantation. Before transplantation the vascular morphology and histology was observed, and respectively in vascular transplantation after7days,14days,28days,42days, selected experimental animals6of each group randomly porting lateral femoralartery color doppler ultrasound examination and histopathologic examination, statisticsgrafts patency rate, observe the transplant vascular anastomotic thrombosis and graftsperiod of histological changes. Transplanting animal groups in terms of the lateralfemoral artery cumulative patency rate are compared, and two inspection standard for P <0.05was statistically significant.Results：The patency rate of each group is l00%immediately after the completion ofvascular transplantation,(decision criteria for transplantation to complete distal femoralartery pulsation can be hit or filling is good, filling poor as a consistent failure, notincluded in the experimental group). The experimental group patency rate varies bytransplant gradually decreases with the extension of time. Vascular transplantation after6weeks, group A cumulative patency rate was87.5%, B group was70.8%,79.2%, group Cgroup D is62.5%; Group A and group C, group A and group B, P>0.05, the difference isnot significant. Group D compared with A, B, C,P <0.05, the difference was statisticallysignificant. For group A fresh autologous vascular transplantation group was the highestcumulative patency rate, the lightest inflammation degree. D group stored in48hoursallograft vascular transplantation patency rate is the lowest, tissue inflammation reactionfrom group A, B, C, anastomotic stenosis degree, transplant vascular intima and middlesmooth muscle hyperplasia, vascular inflammation around is more serious. Group B andC can sustain higher transplantation patency rate,4and6weeks after transplant vascularintima only micro thrombosis, inflammatory cells infiltration and hyperplasia of smoothmuscle cells are mild.Conclusions：Fresh blood vessels in4℃after in vitro preservation of allograft vasculartransplantation is feasible, its time to24hours as the best preserved, transplantation usingheparin can reduce the period of vascular thrombosis and obviously increase the patencyrate.