| Objective:To construct an animal model of acute spinal cord injury of S-D rats, and toobserve the motor function recovery, neuroprotective effect of combination with BBGand low dose methylprednisolone of SCI rats.Method::1）experimental animal grouping：52male SD rats were randomized intofive groups: a sham operated group, a trauma control grope, a BBG group, a high doseMP group, a BBG combined with low dose MP group.2)SCI animal model establishment: The rats under anaesthesia to expose the T1O spinalcord, utilizing a impactor rod weighing10g freely falling from12.5mm to impact theT10spinal cord to establish SCI model.3)Observation items:(1)BBB motor function score system was applied to assess themotor function change of rats at1d、3d、7d、14d、21d、28d、42d after SCI.(2)Thesamples of spinal cord were obtained at3d and42d after SCI. Utilizing HE dyeing toobserve the pathological changes and LFB dyeing to observe the area of remainedspinal cord white matter.(3)Immunohistochemistry was used to detect the change ofGFAP positive cells.Results:1)The BBB score of group A was21,showed no difference with preoperation.The BBB score of the remaining4groups was0at1d after SCI, then the BBB scorerecovered at different levels. The BBB score of group B,C,D,E was8.25±0.957,10.67±0.816,12.00±0.632,12.50±0.837respectively at42d after SCI. The BBB scoreof group B compared with group C,D,E have significant statistical difference(P<0.01)respectively. The BBB score of group C compared with group D and E also showstatistical difference(P<0.05) respectively. There was no statistical difference betweengroup D and E(P>0.05). 2)HE dyeing observation: The injured region of spinal cord appeared disorderstructure,diffuse hemorrhage, exudation, necrosis, swelling and a great quantity ofmononuclear or polynuclear inflammatory cells infiltration at3d after SCI. Thepathological changes had no significant difference between all groups at3d after SCI.The injured region of each group showed different levels regeneration change at42dafter SCI, among which group D and E were better than group B and C.3)LFB dyeing observation: The white matter of spinal cord in group A was stained withsymmetrical blue color, without demyelination and the area of remained spinal cordwhite matter was2.175±0.050mm242d after operation. The remained groups showeddifferent levels demyelination and decreased blue color area of spinal cord white matterat42d after SCI, among which group B showed the least remained spinal cord whitematter area and group D and E showed more remained spinal cord white matter area.The group B compared with group C,D,E have statistical difference(P<0.05)respectively. The group C compared with group D and E also show statisticaldifference(P<0.05) respectively. There was no statistical difference between group Dand E(P>0.05).4)GFAP Immunohistochemistry observation: The number of GFAP positive cellsincreased in the injured region of each group at3d after SCI. There were a great numberof GFAP positive cells in the injured region of group B. Though compared with groupA,the number of GFAP positive cells also increased in group C,D,E, not so obviousthan group B. The number of GFAP positive cells showed no obvious difference amongeach group.Conclusion:1)BBG combined with low dose MP can promote motor functionregeneration and reduce the pathological changes of rats after SCI.2)BBG combined with low dose MP can depress demyelination of spinal cord whitematter after SCI.3)The neuroprotective effect on rats after SCI of combination utilize with BBG and lowdose MP may be related to depressing the glial scar in the injured region of spinal cord. |
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