Evaluating The Defibrase Treatment Effect Of Batroxobin For Deep Vein Thrombosis Of Rat

Part oneObjective:to ensure that batroxobin has a fibrinogen-degrading effect.Methods:We made a self-control experiment on the SD rat (Hemospasia from heart twice,2.7ml blood was adopted each time and then put it into a vacuum tube loading with0.3ml natrium citricum).The interval between the two blood adoptions is one week(7days),and one hour before the second blood adoption we injected batroxobin (a dose of2BU/kg)into the rats intravenously as experimental group. We detected the fibrinogen levels with full automatic blood coagulation analyzer(STAGO),and analysed the difference between the control group and experimental group.Result:After autologous injected batroxobin, fibrinogen levels had been significantly lower, the difference has statistical significance(p=0.00). Studies were done in Spraque-Dawley (SD for short)rats (250to300g) and we used3%Sodium Pentobarbital as anesthetic (Intraperitoneal injection of anesthesia).Finally,we use SPSS19.0for date analysis.Conclusions:Batroxobin has a obviously fibrinolytic effect. Part twoObjective:To establish an animal model of deep vein thrombosis(DVT for short),and make sure whether the result of DVT has a statistical difference between batroxobin group and the control group. Methods:According to the literature search and consider to the actual situation,deep vein thrombosis models were produced by ligating the inferior vena cava (IVC for short) and its branches between bilateral renal vein and bilateral iliac vein for30minutes. We set the experimental group and the control group differently,the former was built by tail-vein injection of2BU/kg batroxobin,while the latter was injected with equal dose of Physiological saline instead. Analysising the outcomes of the control group and the experimental group to ensure whether there was a significant difference between them or not.Studies were done in Spraque-Dawley (SD for short)rats (250to300g) and we used3%Sodium Pentobarbital as anesthetic (Intraperitoneal injection of anesthesia).Finally,we use SPSS19.0for date analysis.Results:Compared to the control group,the thrombosis formation rate of experimental group has dropped,and the difference had statistical significance(p=0.042).Conclusions:Batroxobin may has the effect of preventing DVT formation. Part threeObjective:To study the bleeding risk of batroxobin on colorectal surgery in rat model.Methods:Deep vein thrombosis models were produced by ligating the inferior vena cava and its branches between bilateral renal vein and bilateral iliac vein for30minutes. We set the experimental group and the control group differently,the former was built by tail-vein injection of2BU/kg batroxobin,while the latter was injected with equal dose of Physiological saline instead. In order to calculate the amount of bleeding in different groups, we used small pathological specimen bags to seal blood-stained cottons and weigh the cotton-loaded specimen bags before and after colorectal surgery for every one. Made a statistical analysis of difference between the two groups. Studies were done in Spraque-Dawley (SD for short)rats (250to300g) and we used3%Sodium Pentobarbital as anesthetic (Intraperitoneal injection of anesthesia).Finally,we use SPSS19.0for date analysis.Results:Bilateral inspection result is:P=0.071, namely the use of batroxobin group and the saline group had no significant difference compared with the amount of bleeding.Conclusions:Batroxobin has little bleeding risk during colorectal surgery using2BU/kg dose.