The PCSK9Gene RS11206510Polymorphisms And Atorvastatin Treatment Efficacy With Coronary Heart Disease

Objectives:Cholesteryl ester transfer protein and its main function are to promote the exchange and balance of cholesterol esters and triglycerides in plasma lipoproteins. Related to HDL and LDL particle size and lipid composition plays a key role in reverse cholesterol transport. Recently, The research results are still controversial whether Plasma PCSK9mRNA levels and HDL-C and increase HDL-C levels inhibit PCSK9activity can reduce CHD prevalence rate.This study investigated the polymorphism of the China Tianjin crowd PCSK9gene rs11206510SNP polymorphism, PCSK9and lipid metabolism and the risk for CHD research; PCSK9rs11206510genotypes observed statins in patients with coronary heart disease lipid role.Methods:1. Select778patients who were admitted to coronary angiography in Tianjin Chest Hospital from Sep2010to Oct2011.According to the results of coronary angiography classified into CHD group, n=484,normal control group,n=294.Application of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to the total research object rs11206510gene loci polymorphisms were analyzed,at the same time by using Real-time PCR for determining the content of the serum PCSK9mRNA experssion levels,Analysis of the PCSK9and lipid metabolism and the correlation of CHD risk.2. CHD patients admitted to hospital for atorvastatin calcium20mg, every night. After treatment for3months, observation of statins to carry the PCSK9RS11206510T genotypes of coronary heart disease in the blood lipid regulation.Results:Tianjin area the Han population PCSK9gene rs11206510sites allele frequency are0.110, significantly less than the allele frequencies of European(0.250).CHD group TT, TC genotype compared serum HDL-C levels upward trend, the difference was statistically significant (P<0.05). CHD group in TT, TC genotype comparison, serum TG, CHOL, LDL-C,VLDL-C,ApoAl,ApoB level upward trend, were statistically significant difference (P<0.05). CHD group and the control group PCSK9rs11206510genotypes and allele distribution differences were statistically significant (P<0.05). PCSK9rs11206510genotypes are associated with an increased risk for CHD.and PCSK9rs11206510genotypes may be the susceptible gene in patients of Tianjin area with CHD. There are linkage disequilibrium between TT and TC genotype vis linkage disequilibrium analysis.Diabetes, smoking history, serum LP (a), ApoB risk factors for CHD, Serum HDL-C protection factors for CHD. Serum TG, CHOL,LDL-C and LP (a) level of TC genotype are obviously lower than TT genotype. The rate of decline are23.4%,21.7%,35.7%and17.6%.and Serum HDL-C and ApoAl of TT genotype is the biggest movers with the rate of rise are22.7%and24.8%.Conclusions:Tianjin Han population PCSK9rs11206510genotype and allele frequency distribution are far lower than average of European. HDL-C levels of patients with PCSK9rs11206510genotype are significant increase. PCSK9rs11206510genotype may be the susceptible gene in patients of Tianjin area with CHD. There was a correlation between PCSK9rs11206510gene polymorphisms and atorvastatin calcium CHD patients with lipid regulating role.TC genotype is more powerful for the treatment of levels of TG,CHOL,LDL-C and LP(a).and TT genotype is more powerful for the rise of HDL-C and ApoAl.