Study On Antioxidative Activity Of The Fermented Rhodiola Rosea

ObjectiveIn recent years, with the rapid development of science and technology, people found that more than90%of the disease closely related with active oxygen and free radical. Rhodiola rosea belongs to the Crassulaceae subfamily Sedoieae Berger, Rhodiola l. Rhodiola mainly in Europe, Asia especially in alpine zone of Siberia and North America. In Asia at least more than20kinds of Rhodiola root used in traditional medical system, including R.Sachalinensis, R.Alterna, R.Crenulata, R.Kirilowii, R.Rosea, R.Quadrifida, R. sacra。Rhodiola rosea contained polyphenols flavonoids Saponins, polysaccharides and many other functional components and mineral elements. It was high nutritional value and deeply loved by the people. In recent a few years, domestic and foreign scholars confirmed through a lot of research:Rhodiola could protect liver, anti tumor, enhance immunity and had anti-oxidation effect. But not yet active Yeast fermentation study on anti-oxidation of Rhodiola components. This experiment through Yeast fermentation study on anti-oxidation effects of Rhodiola celestial bodies within and outside the, filter out the strongest anti-oxidant function of fermentation conditions, provided for the development and utilization of Rhodiola functional theory.Methods①Take Rhodiola cut,60℃dried, crushed, and have60heads sieve. Take200g wheel coarse powder extract of Rhodiola fermented (yeast) extract of non-fermented Rhodiola.②In vitro experiment, using DPPH radical scavenging capacity, oxygen radical absorbance capacity (ORAC), and cells antioxidant activity (CAA) experiment, selected functional components with optimal antioxidant capacity.③In vivo experiment,90mice were randomly divided into blank control group, model group, positive control group, the fermented Rhodiola of low, medium and high dose groups. It was10mice in each group. Rhodiola is not fermented of low, middle and high dose groups, were respectively given drugs200mg/kg·BW,400mg/kg·BW,800mg/kg·BW dose, once a day; black control group and model group were gavaged dH2O 0.1mL/10mg·BW daily; positive control group were gavaged Vit C10mg/kg·BW daily. It was gavaged for30days. After the last gavage, in addition to the black control group, the other8groups fasted for16h (overnight), and then lavaged1time with50%ethanol12mL/kg·BW. The mice were recovered feed and water ad libitum. After6h, take eye blood supernatant of the mice. Use enzyme-linked immunosorbent assay (ELISA) detection in serum of mice, glutathione (GSH) activity, protein carbonyl (PCO),8-hydrogen-oxygen ISO prostaglandin (8-iso-PGF2α) contents and superoxide dismutase (SOD), heme oxygenase-1(HO-1), NADPH quinone oxidoreductase (NQO-1) expression. NF-E2-related factor2(Nrf2)Results①The DPPH radical scavenging activity results showed that:the fermented Rhodiola group and non-fermented Rhodiola group DPPH· scavenging activity was significantly higher than fermented and non-fermented Rhodiola+Ginseng Ginseng group (P<0.01). Comparison of fermented and non-fermented fermented and non-fermented Rhodiola+Ginseng Ginseng group DPPH· scavenging ability, were no significant difference (P>0.05). The fermented Rhodiola group and non-fermented Rhodiola group (P<0.01或P<0.05).The fermented fermented Rhodiola group IC50value was80.968μg/mL μg/mL, non-fermented Rhodiola group IC50value was158.830u g/mLμg/mL. Their IC50values were greater than ascorbic acid group (40±0.01) μg/mL.②The oxygen radical absorbance capacity results showed that:the fermented Rhodiola group oxygen free radical absorption capacity was significantly higher than that of non-fermented Rhodiola group, fermented and non-fermented Rhodiola+Ginseng Ginseng group (P<0.01). fermented and non-fermented Rhodiola+Ginseng Ginseng group were compared, absorption capacity oxygen free radicals without significant difference (P>0.05).③The intracellular antioxidant activity results showed that:the each dose group of fermented Rhodiola group and non-fermented Rhodiola group, cell fluorescence intensity compared with the black control group, the difference was statistically significant (P<0.05or P<0.01); Fermented and non-fermented Rhodiola+Ginseng group of concentration150μg/mL,200μg/mL,250μg/mL dose group compared with the black control group, there were significant differences (P<0.05). Fermented Rhodiola group inhibition of ROS oxidation of DFCH (P<0.05) had the strongest ability.④The fermented Rhodiola of low, middle and high dose group, the activity of GSH were significantly higher than that in model group (P<0.05or P<0.01).⑤The fermented of Rhodiola of low, middle and high dose group, PCO and8-iso-PGF2α contents were significantly lower than that in model group (P<0.05or P<0.01).⑥The fermented Rhodiola of low, middle and high dose groups, SOD, HO-1and NQO-1expression were significantly higher than that in model group (P<0.05or P<0.01).⑦The fermented Rhodiola high dose group and positive control group, comparison of GSH activity, PCO、8-iso-PGF2α contents and the expression of SOD、HO-1、 NQO-1,Nrf2,the difference was not statistically significant (P>0.05).Conclusions①Fermented, fermented Rhodiola and non-fermented Rhodiola have antioxidant activity. Fermented of fermented Rhodiola antioxidant activity were better than that of the non-fermented Rhodiola, fermented and non-fermented Rhodiola+Ginseng, fermented and non-fermented Ginseng②Fermented Rhodiola can enhance the activity of antioxidant enzymes, reduce the protein oxidative damage and lipid peroxidation damage in vivo, and promote antioxidant protein expression.