| 1. Background and ObjectiveThe concept of Non-alcoholic fatty liver disease (NAFLD) refers to a wide rangeof diseases in liver, including simple fatty degeneration and non-alcoholicsteatohepatitis (NASH). It has been identified as the most common chronic liverdisease. NAFLD is a component of the metabolic syndrome (MS), and is consideredto be a risk factor for cardiovascular disease. Its main pathological change is theaccumulation of fat in the liver, but It may lead to cirrhosis and even hepatocellularcarcinoma. Therefore, NAFLD can be considered as a risk factor of cancer.Considering its rising incidence and adverse consequences, it is very important toimprove our awareness and clinical measures to cope with it. The clinical diagnosis ofNALFD is mainly relied on ultrasonography. However, the result is influenced by thesubjective factors of the operator, such as the experience and the standards ofcertainty. The lipid parameters of TG and LDL-C also were not a good indicator ofthe severity of NALFD due to they were easily influenced by multiple factors. Byfeeding a high-fat&high-sugar diet to rats, we copied the model of non-alcoholicfatty liver disease (NALFD) in order to explain the role of Apolipoprotein B100(ApoB100), insulin resistance and hypertension in the progress of NALFD. Coronaryheart disease is frequently associated with hypertension in clinical. Isosorbidemononitrate (ISO) is a commonly used drug to dilate the coronary vessels. The studyalso observed the impact of ISO on the blood lipid and glucose in rats withhypertension and non hypertension. 2. Objects and Method2.1Grouping and fodder12weeks-aged male spontaneously hypertensive rats（SHR）and Wistar rats were(represented respectively by S and W) randomly divided into normal dietgroup(normal W, normal S), high-fat group(Fat W, Fat S) and ISO interventiongroup(ISO W, ISO S). Normal diet group was fed with conventional diet, high-fatgroup was fed with high-fat&high-sugar (HFHS) diet. ISO group was fed withHFHS diet plus ISO intervention.2.2Observing time and indexAfter12weeks of feeding, ApoB100, serum triglyceride(TG) and totalcholesterol(TC) were measured; IVGTT and IVIRT were carried out, then calculatedthe insulin resistance index（HOMA-IR）. The severity evaluation of NAFLD basedon the activity score with the light microscope observing after HE staining of livertissue.2.3Statistics AnalysisThe relationships among the severity of NAFLD and the levels of ApoB100, TG,TC and HOMA-IR were analysis by SPSS software. We also evaluated the impact ofISO intervention on blood lipids, HOMA-IR and NAFLD.3. Results3.1After12weeks，Comparing in the same species of rats，the values ofApoB100, TG, TC, HOMA-IR in HFHS groups and ISO groups were higher thannormal diet groups, and the difference was statistically significant(p <0.05);3.2ISO S group compared with Fat S group, the HOMA-IR is higher andNAFLD much more serious. The NAFLD in Fat S group more serious than in fat Wgroup, the differences were statistical significance (p<0.05)3.3Correlation analysis showed that there was a high correlation betweenApoB100and the severity of NAFLD(r=0.918,p=0.010）, its r value is higher thanTC(r=0.903, p=0.014), LDL-C(r=0.871, p=0.024) and HOMA-IR (r=0.812, p=0.033).4. Conclusions4.1The insulin resistance is occurred in Wistar rats and SHR rats after12weeksHFHS feeding. HOMA-IR is positively correlated with the severity of fatty liver.4.2SHR rats were more likely suffer from NAFLD; 4.3The ApoB100values is positively correlated with the severity of NAFLD.4.4ISO intervention increases the degree of NAFLD in SHR rats, but have nosignificant effect on Wistar rats. The reason is not known. |
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