Study Of Genetic Susceptibility In135Children With Asthma

Bronchial asthma is a common chronic respiratory disease with serious damageto patients’ health. it has a high incidence and often be characterized by recurrentepisodes of chronic course,in addition,it has a serious impact on the patients’ learning,life and physical activities, affecting the growth and development of children andadolescents. Due to improper treatment time,many children with asthma finallydevelop into asthma in adults and be persistent,even seriously damage lung function,some patients even completely lost his ability to do physical activities.Therefore,timely and accurate diagnosis of childhood asthma for children with asthma have aimportant significance. Childhood asthma gene is currently a research focusing on thechildhood asthma, through the analysis of gene can not only assess perspective ofgene genetic risk of childhood asthma, but also will improve the diagnosis rate ofchildhood asthma. In order to further understand asthma related genes in thedistribution of asthma children and healthy children, this experiment is designed. ObjectTo discuss the frequency distribution of single nucleotide polymorphisms(SNPs) of four asthma-related gene loci in asthmatic children，and to investigate itsassociation with genetic susceptibility to childhood asthma and some clinicalphenotypes of asthma.MethodFluorogenic quantitative PCR and sequencing technique were used to detect thefrequency distributions of the SNPs of the four asthma-related gene loci in135asthmatic children and98healthy controls．Results1.genotype DD of angiotensin-converting enzyme (ACE) had a significantlyhigher frequency in the asthmatic children than in the healthy controls (χ2=26.475，P＜0.01)，and the frequency of D allele was also significantly higher in the asthmaticchildren than in the healthy controls (χ2=24.242，P＜0.01);2.The genotype AG of Adrenaline receptor beta2subtypes (ADRB2) had asignificantly higher frequency in the asthmatic children than in the healthy controls(χ2=22.505，P＜0.01),and the frequency of allele was also significantly higher in theasthmatic children than in the healthy controls (χ2=6.759，P＜0.01)． Conclusion1.Genotype DD of ACE and genotype AG of ADRB2are related to geneticsusceptibility to childhood asthma and may be the risk factor for childhood asthma;2.Other two asthma genes involved in this work are not be able to repeat.