The Role Of CHD1L In Human Embryonic Stem Cells Self-renewal

Human embryonic stem cells(hESCs) are potential cell resourses in regeneration medicine research because of its pluripotency and continuous self-renewal abilities. But the mechanisms of their self-renewal and pluripootency far from clear. In rencent years, more and more studies focused on the role of chromatin remolding factors in maintaining hESCs self-renewal and pluripotency. CHDIL was previously found as a candidate oncogene in hepatocellular carcinoma, and was found to be involved in a series of cellular processes, such as chromatin remodling, DNA repair, cell growth and survival. CHDIL was also found highly expressed in mouse inner cell mass(ICM) and embryonic stem cells. However, the role of CHDIL in hESCs self-renewal and pluripotency remains unclear. In this study, we investigated the function of self-renewal, cell growth and transcription of pluripotency associated genes by CHDIL overexpression and knockdown.Our study discovered that:1.CHDIL protein is highly expressed in hESCs but not in human embryonic fibroblast; and CHDIL protein is gradually decreased during differentiation of hESCs;2. both overexpression and knockdown CHD1L in hESC resulted in hESCs growth inhibition;3. overexpression CHD1L in hESCs also lead to the decline of pluripotency and self-renewal associated genes expression. In summary, our study found that CHD1L played a very important role in hESC self-renewal. Both CHD1L overexpression and knockdown would hamper hESC self-renewal, and the detailed mechanism is still needed further study.