The Role Of Wogonin In Inhibiting Cell Growth Of Osteosarcoma MG-63Cells And Related Mechanism

Objective:To investigate anti-proliferation and apoptosis inducing effect of wogonin on MG-63cells and its possible mechanism.Methods:Different concentrations of wogonin were given to cultured MG-63cells. CCK-8assay was used to detect proliferation inhibition rate. The DAPI staining was used to observe the cellular apoptosis rate after exposed to a series of concentrations of wogonin. Western blot was used to detect the levels of PARP(a kind of cellular apoptosis protein). Western blot was used to detect the levels of AMPK, S6K and4EBP1phosphorylation in MG-63cells exposed to different time and concentrations of wogonin.Results:CCK-8assay showed that wogonin can inhibit the proliferation of MG-63cells dose-dependently. DAPI staining demonstrated that the cellular apoptosis rate rised up, accompanied with the increase of wogonin concentration. Under certain drug concentrations, wogonin can promote the expression of PARP of MG-63cells. Western blot results showed that, compared to the control group, wogonin can significantly promote the AMPK phosphorylation of MG-63cells, and inhibit the S6K and4EBP1phosphorylation of MG-63cells.Conclusion:Wogonin can inhibit the proliferation and induce apoptosis of MG-63cells, whose mechanism may be regulating AMPK/mTOR signaling pathway.