The Clinical Study Of MiR-17-92Expression In Peripheral Blood Of Sjogren’s Syndrome Patients In Syndrome Of Deficiency Of Yin And Correlation With The Expression Levels And TCM Syndrome Type

ObjectiveSjogren’s syndrome (SS) is a chronic autoimmune disease with a broad clinical spectrum that characiterized of lymphocyte proliferation and progressing organ-specific exocrinopathy to systemic manifestations. The pathogenesis of organs involved in SS we could see lymphocytes infiltration, lots of autoantibodies detected in serum and accompanied with hyperglobulinemia, indicated immunocyte function disorder.MicroRNAs(miRNAs) as small, single-stranded noncoding RNAs, many of whichhave been highly conserved throughout evolution, is known to regulate cellular processes such as growth, development, aging and apoptosis,and associated with human diseases. The miRNAs have multiple regulating potential to broadly influence human immunocytes, and could control the development and function of innate and adaptive immune response by regulating target mRNA expression, and involved in the pathogenesis of autoimmune diseases. In SS the immunocytes aggregation as the peripheral blood mononuclear cell(PBMC), is the essential target to exploring pathogenesis of the disease. To date, little is known about the roles of miRNAs in pSS pathogenesis and few studies had been published about it. Based on this point, this study was comparing the PBMC of pSS patients with healthy controls (Health Control, HC) to explore which miRNA expression profiling changes and impacts on the significance for the pSS. Our aim was to analysis microR-17-92expression profile in PBMC of patients with SS,searching for the special miRNAs associated with SS and investigate the relative expression of special miRNAs of relevance to pSS clinial spectrum. Furthermore, to explore miRNAs which regulate the clinical datas of TCM syndrome of defientcy of YIN.MethodsThe expression level of miR-17-92(including miR-17, miR-18a/b, miR-19a, miR-20a, miR-19b-1, miR-92-1was detected by qPCR method in larger sample size,including20cases of patients with SS and10cases of healthy controls. We analysised the expression levels of those miRNAs with the pSS patients’organ involvement condition, disease activity index and laboratory tests (IgG, ESR and the titre of ANA),and the ESSDAI scroes, and to investigate the relationshipi between the expression levels of miRNA-17-92and clinical datas of TCM syndrome of defientcy of Yin.Result1.miR-17,miR-19a, miR-20a was detected upregulated in SS patients’PBMC by qPCR method in an expanded sample size and the relative expression to HC is2.99,1.559,1.490,wichi is with statistical significant difference(P<0.05).2. The analysis of spectrum correlation with clinical profiles shown that miR-17、miR-19a was positively related to the titre of ANA(r=0.398),High expression of IgG, and the classification of Pathological exam of lip;miR-17-92has no mean to compare with scores of ESSDAI.3.miR-17, miR-19a was related to the clinical datas of TCM syndrome of defientcy of YIN, miR-17a,miR-19a has a positive relation(r=0.441,r=0.351).Conclusion:1.miR-17,miR-19a, miR-20a was detected upregulated in SS patients’PBMC by qPCR method in an expanded sample size and the relative expression to HC is2.99,1.559,1.490, which is with statistical significant difference.2. The analysis of spectrum correlation with clinical profiles shown that miR-17、miR-19a was positively related to the titre of ANA, expression of IgG, and the classification of Pathological exam of lip, which suggest that they are very helpful to determine the disease aetivity;3.miR-17, miR-19a was related to the clinical datas of TCM syndrome of defientcy of YIN, miR-17、miR-19a has positively relation, these datas can provide are ference for the objectively research about traditional Chinese medicine syndrome type.