Development Of Allergic Rhinitis Model In129Sv Mice And The Expression Of H2-Eb1Gene In The Nasal Mucosa Of Allergic Rhinitis’ Mice

Objective:(1) The present study aimed to develop allergic rhinitis model in129Sv mice;(2)Investigate the relationship of H2-Ebl gene (orthologous gene of human HLA-DRB1in Mice)with allergic rhinitis pathogenesis in AR mice.Method:(1)368-week old female129/sv mice were randomly divided into Experimental and Control groups (18mice for each Group). All mice were sensitized with ovalbumin (OVA) to establish allergic rhinitis mouse model. After successful induction,nasal mucosa was fixed and stained for pathologic analysis,such as eosinophil (EOS) evaluation and mast cell infiltration etc. The cytokine IL-4,IFN-y and the OVA-specific IgE levels were detected by ELISA method;(2)The H2-EB1protein expression in the nasal mucosa was detected via the immunohistochemistry,immunofluorescence technique as well as western blot. The expression of H2-Ebl mRNA in nasal mucosa was analyzed by quantitative Real time PCR. Result:(1)After the development of AR models in129Sv mice,Compared with the control group,the cilia layer in nasal mucosa of the Experimental group was found partially desquamated; eosinophil (EOS) and mast cell infiltration were found evident in submucosal; Serum OVA-IgE and IL-4level significantly increased, together with the decreased of IFN-y level (P<0.05);(2)The expression of H2-Eb1mRNA and H2-EB1protein were upregulated in the nasal mucosa. Conclusion:(1Successfully developped a stable experimental129Sv mouse model of allergic rhinitis mice which could appear Thl/Th2cytokine imbalance;(2)H2-Ebl mRNA and H2-EB1protein expression in nasal mucosa were significantly upregulated in our successful established allergic rhinitis mouse model,suggesting that HLA-DRB1may play an important role in the pathogenesis of allergic rhinitis in the Thl/Th2balance regulation.