Ox-ldl Induced Lectin-like Oxidized Low-density Lipoprotein Receptor-1Expression In Hepatic Sinusoidal Endothelial Cells And Its Mechanism

Objective:The aim of this study was to investigate the presence of lectin-like oxidized low-density lipoprotein receptor-1(LOX-1) on cultured human hepatic sinusoidal endothelial cells (HHSECs), and meanwhile study the expression regulation of LOX-1by oxidized low-density lipoprotein (ox-LDL) and the potential mechanisms in cultured HHSECs. Methods:Using PCR and western blotting detected whether LOX-1express in the HHSECs. Cells were treated with different concentration ox-LDL (20,40,60,80and100ug/mL). After incubation of HHSECs for12,24,48h, MTT assay were used to analyze the effect of ox-LDL on HHSECs viability. The expressions of LOX-1mRNA and proteins were determined by real-time polymerase chain reaction (RT-PCR) and western-blotting respectively. The intracellular reactive oxygen species (ROS) was monitored by flow cytometry with fluorescence probe. DCFH2-DA. The effect of polyinosinic cytidylic acid (250ug/mL) on ox-LDL induced LOX-1expression was also investigated. Results:LOX-1expressed at both mRNA and protein levels in HHSECs. The cell viability were markedly decreased when HHSECs were treated with different concentration ox-LDL for12,24,48h (P<0.05), and ox-LDL significantly induced LOX-1expression at both mRNA and protein levels in a time-dependent and dose-depended manner compared with control group (P<0.05). However, polyinosinic cytidylic caid restrained ox-LDL induced LOX-1expression and intracellular ROS production (P<0.05). Conclusion:LOX-1exists in HHSECs, and ox-LDL induced LOX-1expression and ROS production. Ox-LDL induced LOX-1and ROS expression through LOX-1signaling pathways.