Comparison Of The Effect Of Astragalus Polysaccharide And Thrombopoietin On In-vivo And In-vitro Hematopoiesis

Objective:To assess and compare the in vivo and in vitro effect of Astragalus Polysaccharide (ASPS) and thrombopoietin (TPO) on hematopoiesis and explore its mechanism.Methods:Myelosuppression mouse model was treated with ASPS (10mg/kg/day) or TPO. Peripheral blood cells from ASPS and thrombopoietin-treated samples were collected and counted on days0,7,14and21.CFU-GM、CFU-MK、BFU-E/CFU-E and CFU-F assays were used to determine the effects of ASPS on progenitor cells. HL-60cells were cultured with serum free medium to induce cellular apoptosis and treated with or without ASPS. After72h incubation, the number of cells was counted. In addition, Annexin V, Caspase-3and JC-1assays were used to determine the proportions of apoptotic cells.Results:Morphological examination showed that ASPS and TPO treatment significantly protected the hematopoietic cells from the irradiation-induced apoptosis. ASPS and TPO significantly enhanced the peripheral blood cells recovery in vivo. Moreover, ASPS promoted the formation of CFU-GM、CFU-MK、BFU-E/CFU-E and CFU-F. We found that ASPS significantly suppressed caspase3activation, and reduced cell apoptosis in HL-60cells.Conclusions:ASPS and TPO might promote hematopoiesis in the irradiation mouse model. ASPS also promoted hematopoiesis in vitro. It reduced the HL-60cell apoptosis via suppressing the activation of caspase-3.