Effects Of Carbon Dioxide On The Expression Of VEGF-C And MMP9in Human Cervical Cancer Cell Line Hela

Objective Through the establishment of in vitro model of carbon dioxidepneumoperitoneum environment, to examine the expression changes of vascularendothelial growth factor-C (VEGF-C) and matrix metalloproteinase-9(MMP9) inhuman cervical cancer cell line Hela, so as to explore the effects of carbon dioxidepneumoperitoneum environment on the invasion and metastatic ability of cervical cancercell.Methods Human cervical cancer cell line Hela was divided into four groups in vitro: CO2for1h group, CO2for2h group, CO2for3h group and control group. CO2for1h group:The cells were placed in the3L sterile drainage bag, connected the sterile drainage tubewith pneumoperitoneum machine after sealed, adjusted the pneumoperitoneum machinepressure to10mmHg,99.5%medical carbon dioxide gas was piped in, lasted1h, thenconventionally trained24,48,72h. CO2for2h group: According to the above operationventilated with carbon dioxide gas, lasted2h, and then conventionally trained24,48,72h. CO2for3h group: According to the above operation ventilated with carbon dioxidegas, lasted3h, and then conventionally trained24,48,72h. Control group: Placed innormal incubator for24,48,72h. The mRNA and protein expressions of VEGF-C andMMP9of these cells were examined by real-time RT-PCR and immunocytochemistry.Results (1) The mRNA and protein expression levels of VEGF-C of CO2for1h group,CO2for2h group and CO2for3h group were significantly higher than control group(P<0.01).(2) With the cultured time of CO2for1h group, CO2for2h group and CO2for3h group prolonged, the mRNA and protein expression levels of VEGF-C reached apeak at24h, followed by a downward trend, but the expression levels were still higherthan control group (P<0.01). With the cultured time of control group prolonged, themRNA and protein expression level of VEGF-C showed no significant change (P>0.05).(3) At the same cultured time, with the role of the time of carbon dioxide prolonged, themRNA and protein expression levels of VEGF-C gradually increased, reached a peak at2h, and then gradually decreased, but the expression levels were still higher than CO2for1h group (P<0.01).(4) The mRNA and protein expression levels of MMP9of CO2for1hgroup, CO2for2h group and CO2for3h group were significantly higher than control group (P<0.01).(5) With the cultured time of CO2for1h group, CO2for2h group andCO2for3h group prolonged, the mRNA and protein expression levels of MMP9gradually increased, reached a peak at48h, followed by a downward trend, but theexpression levels were still higher than24h (P<0.01). With the cultured time of controlgroup prolonged, the mRNA and protein expression level of MMP9showed nosignificant change (P>0.05).(6) At the same cultured time, with the role of the time ofcarbon dioxide prolonged, the mRNA and protein expression levels of MMP9graduallyincreased, reached a peak at2h, and then gradually decreased, but the expression levelswere still higher than CO2for1h group (P<0.01).Conclusions (1) Carbon dioxide pneumoperitoneum environment of vitro couldupregulate the mRNA and protein expression of VEGF-C and MMP9of Hela cell.(2)With the role of the time of simulated carbon dioxide pneumoperitoneum environmentprolonged, the mRNA and protein expression levels of VEGF-C and MMP9graduallyincreased, showed a downward trend after the peak.(3) With the cultured time prolonged,the mRNA and protein expression levels of VEGF-C and MMP9gradually increased,showed a downward trend after the peak.