The Feasibility Study Of Tissue Engineering With Human Urine-derived Stem Cells

Objectives To compare the capacity of induced differentiation between urine-derived stem cells and urine-derived induced-pluripotent stem cells, the feasibility of two sorts of cells applying in the tissue engineering was discussed. Smooth muscle cells derived from urine stem cells were used to get the multi-layer cell sheet with the use of Nunc Upcell dishes.Methods Urine-derived stem cells was induced towards three different directions of smooth muscle cells, endothelial cells and nerve cells, and was tested the expression of specific mRNA and proteins with the use of qRT-PCR and immunofluorescent staining. Transferring the induced smooth muscle cells into Nunc Upcell dishes, we can acquired the single-layer cell sheet. And then multi-layer cell sheet was gained by overlaying single-layer cell sheet several times. Urine-derived induced-pluripotent stem cells was also induced into three different directions, and was tested the expression of specific mRNA and proteins with the use of qRT-PCR and immunofluorescent staining. By comparing the induction effects, the feasibility of two sorts of cells applying in the tissue engineering was discussed.Results (1) During the process of smooth muscle cells and endothelial cells induction of urine-derived stem cells, the expression of pluripotent gene was declining relatively, while the expression of specific markers of smooth muscle cells and endothelial cells was increasing clearly.(2)During the process of nerve cells induction of urine-derived stem cells, the expression of gene was not fluctuated fiercely except Nestin.(3)The multi-layer cell sheet was gained by using USC-SMC combining with Nunc Upcell dishes.(4)The induced potency of iPSCs is better than USCs in different directions. Neural progenitor cell specific structure rose set can be seen in the iPSC-NC. The expression of neural specific gene was increasing during the neural induced process, meanwhile the expression of Sox2gene maintained in a high level.Conclusions This research identified the fact that the induction effect of USCs to SMC and EC is better than NC. The multi-layer cell sheet was gained by using USC-SMC combining with Nunc Upcell dishes. By comparing the induction effects between USCs and iPSCs, this research identified the fact that iPSCs had a better induced potency and had a better prospect in tissue engineering.