FISH Detection Of HTERC Genes In The Clinical Significance Of The Early Screening For Cervical Cancer

Objective：The application of fluorescence in situ hybridization (FlSH) is to detect thetelomerase genes of the expression and correlation of cervical intraepithelial neoplasia andcervical carcinoma HTERC genes is to assess the clinical significance of the detection ofHTERC genes by the cervical lesion screening.Methods：The240cases of cervical lesion patients from May,2012to May,2013inGeneral Gynaecological Centre affiliated Ningxia Medical University were randomlycollected. All patients were did liquid-based cytology (TCT) inspection, high risk HPVtesting,colposcopy and pathological diagnosis. TCT is divided into the following typesaccording to cytology:46cases of no epithelial within tumor NILM,62cases of ambiguousatypical squamous cells (ASCUS),46cases of low-grade squamous intraepithelial lesion(LSIL),68cases of high-grade squamous intraepithelial lesion (HSIL),18cases of cervicalcancer(Scc);154cases of HPV positive,86cases of HPV negative.Organizational pathologicaldiagnosis is divided into:56cases of Cervical Inflammatory Group,70cases of CINⅠ group,86cases of CINⅡ/Ⅲ group28cases of Cervical Cancer Group. At the same time,46NILMwomen were selected as the comparative group in order to establish the experimentalthreshold with the expression of hTERC genes in the group.Results：1. The comparative threshold is8.7%. it means the comparative group occurred inthe number of cells per-patient hTERC genes amplification does not exceed8.7%.2.The TCT grade and the high-risk HPV infection is closely related. The high-risk HPVpositive expression rate was significantly increased with TCT grading increments. The TCTabnormal cytology its detection rate in negative group (NILM), there was significantdifference(P<0.01）.3.The hTERC genes amplification in HPV positive group was75.3%(116/154), while in86cases of HPV negative cases was25.5%(22/86).The comparison between the HPV groupsand non-infected groups shows significant differences of the expansion of HTERC(P<0.01）.4. HTERC genes grouped in cytology NILM group, ASC-US group, LSIL group, HSIL group,SCC group, the positive expression rates were8.7%,12.9%,30.4%,76.5%and100%. HTERC gene amplification severity increases with increasing height gene amplificationlesion group was significantly higher than low-grade lesions group (P <0.01), while thedifference in height lesions and cervical cancer groups was not statistically significant (P>0.05), the study group and the control group hTERC gene expression rate difference wasstatistically significant (P <0.01).5. HTERC genes in Cervical Inflammatory group, CINI group, CINII/III group and the SCCgroup, the positive expression rates were7.14%,37.14%,79.06%and100%. HTERC genesexpression rate varies significantly elevated levels of pathology amplification was statisticallysignificant (P <0.05), and genes amplification hTERC cancer group were significantly higherthan CIN group, there were significant differences between the two groups (P <0.05).Conclusion：1. FISH to detect gene amplification of hTERC can be used as an indicator topredict pre-cancerous lesions of the cervix.2.HTERC genes can be used as one of lesions cervical cancer screening and earlydiagnosis of cervical molecular biomarkers, which can be used as an adjunct to TCTexamination and HPV-DNA testing.3. HTERC genes with significantly increased histopathological grading amplification, sothe positive rate hTERC gene amplification can be used as further evidence of CIN malignantevolution.