Study On Accompanying Material Influence Physiological Disposition Of Saponins Components In Anemarrhena Asphodeloides

Objective By using refining separation technology to obtain different Accompanying Material of Anemarrhena asphodeloides saponins extracts.Study on Accompanying Material Influence Physiological Disposition of Saponins components in A.asphodeloides to provide a basis for refining and separation of traditional Chinese medicine extract.Methods 1.Preparation of different accompanying materials and determination of their total saponins content: Preparation of different accompanying materials timosaponin extract, the determination of their total saponins content.2.The influence of pharmacokinetics profile of differentaccompanying materials from A.asphodeloides extracts in rats. Oral administration with different accompanying materials from Anemarrhena asphodeloides extracts, simultaneous determination of four saponins in rat plasma by LC-MS/MS. 3.Influence of tissue distribution of different accompanying materials from A.asphodeloides extracts in rats: Oral administration with different accompanying materials from A.asphodeloides extract,It simultaneous determination of four saponins in rat tissue by LC-MS/MS. 4.Influence of absorption of different accompanying materials from A. asphodeloides extracts in Caco-2 model: The evaluation of influence of Papp in Caco-2 with different accompanying materials from A.asphodeloides extracts.Results 1.By using Water extract-alcohol precipitation and porous resin column to obtain different accompanying materials from A. asphodeloides extract. Their contents of total saponins was 21.97%,47.28% and 66.97%,respectively.2. Oral administration with different accompanying materials from A.asphodeloides extracts, comparison of pharmacokinetic profile of four steroidal saponins in group simple third. The AUC value of four steroidal saponins greatly improved in group simple second. Cmax and AUC of four steroidal saponins, the major active components of A. asphodeloides increased significantly in group simple first.There are no significant differences among all the groups in Cmax, Tmax, T1/2 and AUC, indicating accompanying materials of simple four have no influences on absorption, elimination and bioavailability of four saponins in rat plasma. 2.Unlike accompanying materials after gavage.The content of four saponins was mainly distributed in liver, however,the content of four saponins prototype in kidney was very poor.In addition, the time to maximum concentration is 0.5 h in every tissue.It indicated that the saponins in A. asphodeloides were distributed very fast.The different accompanying materials had different influence on distribution of every component,and it did not impact these rules.4.The results of Caco-2 modelshowed that accompanying materials in group second and first accompanying material could improve absorption in intestinal of saponins in A.asphodeloides. However, accompanying materials of simple four had no significant influence on Papp in saponins in A.asphodeloides.The efflux ratio of foursaponins was about 1 and less than 1.5,which indicated that these saponins maybe mostly passive diffusion.Conclusion The experiment indicated that the different accompanying materials had different influence on saponins in A.asphodeloides in physiological disposition.The results that macromolecular fraction of A. asphodeloides extracts had no significant impact on saponins in physiological disposition.However,the traditional Chinese medicine in the treatment of disease from the multiway,multitargets,multiposition to play a role,its pharmacological effects are various.Moreover,Combined with its pharmacodynamics and the corresponding animal model to investigate the effect of A.asphodeloides extracts with accompanying materials efficacy of main active components.