The Expression And Significance Of VEGF、 MIF In Colorectal Cancer

BackgroundVascular endothelial growth factor(VEGF) can induce the rebirth of tumor vessel, and the macrophage migration-inhibitory factors(MIF) is a kind of cell factor with multi-biological functions, which can accelerate the hyperplasia of tumor vessel and the spread of tumor. Both VEGF and MIF are two kinds of cell factor with multi-biological functions, and various research have found that VEGF and MIF played an important role in genesis and development, invasion and metastasis of malignant tumor.Through immunohistochemical method testing the manifestation of VEGF and MIF in colorectal cancer and the relationship with clinicopathologic data, the study combined with follow-up data research and relations with patient outcome and helped to explain biological behaviour of colorectal cancer from microscopic aspect. Additionally, the study discussed whether it can serve as the molecular marker of colorectal cancer in early diagnosis, and further provide basis for carrying out the genetic diagnosis and gene therapy of colorectal cancer. ObjectiveTo study the relationship between the manifestation of VEGF and MIF in colorectal cancer and the clinical stages of colorectal cancer, and the influence of patient outcome. MethodsTo analyze and follow up the clinicopathologic data of 80 patients with colorectal cancerinthegeneralsurgerydepartmentofthefirstaffiliatedhospitalofanhuimedicaluniversityretrospectively.totesttheexpressionlevelofvegfandmifincolorectalcancerwithimmunohistochemicalmethodsandanalyzetherelationshipbetweentheinfiltrationandclinicopathologicdataofvegfandmifincolorectalcancerandtheinfluenceofpatientswithcolorectalcancer.results1.thepositiverateofvegfandmifincolorectalcanceris77.50%and82.50%respectively.comparedwiththeregularpositiverateincolorectalcanceris22.50%and27.50%respectively,thepositiverateincolorectalcancerishigherthancontrolgroup,the test of χ2 showed the statistical significance(P<0.05). 2. The expression of VEGF and MIF in colorectal cancer is related to the invasion depth, lymphatic metastasis, clinical stages of tumour(P<0.05), and not related to sex, age, and histological differentiation(p>0.05). 3. The positive rate of infringement serous layer VEGF of colorectal cancer is 80.60%, and the positive rate of non infringement serous layer VEGF of colorectal cancer is 46.51%. The positive rate of lymph node metastasis VEGF is 83.51%, and the positive rate of no lymph node metastasis VEGF is 41.52%. The positive rate of patients with Ducks C/D VEGF is 93.39%, and the positive rate of patients with Ducks A/B VEGF is 38.31%. 4. Through the relevant analysis of Pearson, the expression of VEGF and MIF in colorectal cancer showed positive correlation prominently, and the difference has the statistical significance(r=0.382, P<0.05). 5. Through the follow-up analysis and the comparison between positive patients and negative patients with VEGF and MIF, the survival rate reduced prominently, and the difference has the statistical significance(P<0.05).Conclusions 1. The positive rate of VEGF and MIF in colorectal cancer is higher than the regular large intestine, and patients with lymphatic metastasis are higher than patients without lymphatic metastasis, which showed that VEGF and MIF may improve the genesis and development of tumour and lymphatic metastasis of tumour. 2. The correlation analysis of VEGF and MIF showed that the expression of them has the positive correlation, and hinted them to give play to the synergistic effect of genesis and development, nfiltration and transfer in the tumour. 3. The survival analysis showed that between positive patients and negative patients with VEGF and MIF in colorectal cancer, the survival rate reduced prominently, and hinted that the expression of VEGF and MIF is related to bad prognosis of patients with colorectal cancer.