The Quality Evaluation Research Of Four Root And Rhizome Medicines Of Sll Via Miltiorrhiza, Radix Astragali, Platycodon Grandiflorum And Dioscorea Septemloba

Objective To established for analysis and evaluation of chemical constituents and residues of organochlorine pesticides of roots and rhizomes, including Salvia miltiorrhiza, Radix astragali, Platycodon grandiflorum and Dioscorea septemloba from different habitats. It should provides scientific basis for its quality control.Method: To establish HPLC fingerprint and quantitative determination method for determination of index components of Salvia miltiorrhiza, Radix astragali, Platycodon grandiflorum and Dioscorea septemloba from different habitats. And then further study on the methods of qualitative and quantitative to residues of organochlorine pesticides by GC. To establish a preliminary study to the residue of organochlorine pesticides from Platycodon grandiflorum by Near-infrared Fourier Transfer Raman spectroscopyResults: The HPLC chromatographic fingerprint of chemical constituents were established. The characteristic common peaks were basically identical in these four medicinal materials, but the peak heights and areas were differences. The 14, 13, 12, 15 batches each from Salvia miltiorrhiza, Radix astragali, Platycodon grandiflorum and Dioscorea septemloba. Salvianolic acid B and tanshinone IIA from Salvia miltiorrhiza, astragaloside IV from Radix astragali, deapio platycodin D from Platycodon grandiflorum and diosgenin glycoside from Dioscorea septemloba were identified by retention time of these reference substances. And the content ranges of salvianolic acid B were 3.470~9.320%and tanshinone IIA were 0.071~0.345% with Salvia miltiorrhiza. The content ranges of astragaloside IV were 0.011~0.073% with Radix astragali. The deapio platycodin D content were 0.0403~0.1349% with Platycodon grandiflorum and the diosgenin glycoside content were 0.0190~0.0383% with Dioscorea septemloba. The results of residue of organchlorine pesticide showed the BHC and DDT were detected from these four medicinal materials by GC and the residue of organochlorine pesticides in Radix astragali were less than in the regulation of Chinese pharmacopoeia(2010). The results of residue of organchlorine pesticide in Platycodon grandiflorum by NIRS technology showed that the method should determine their contents. But Platycodon grandiflorum have absorptions in near-infrared spectral regions, the data processing was interfered by these extra information. Therefore it should not determine the contents and kinds with the residue of organchlorine pesticideConclusion: This method was accurate and reliable and it could reflect the integrity and diversity of these four medicinal materials from different habitats. The capillary gas chromatography method was established to detect the residue of organochlorine pesticide and suitable for these four medicinal materials. The temperature gradient is quite gently and the chromatography baseline is straight, it should improve the quantitative accuracy. It should provide the references to studing the residue of organochlorine pesticide by the other medicinal materials. The results detected the residue of organochlorine pesticide of the medicinal materials is no-ideal by NIRS technology, but also it should provide the certain theoretical basis and references for further study. In a word, it provides a scientific basis for quality evaluation standard by the research of chromatographic fingerprints and the residue of organochlorine pesticide from Salvia miltiorrhiza, Radix astragali, Platycodon grandiflorum and Dioscorea septemloba.