| objective The study was accorded to the Atherosclerosis model by SD rates and give the drug intevention,combining HE staining,imumunohistochemistry, Polymerase Chain Reaction(RT-PCR) to oberseve change of the expression of CD105 in the atherosclerotic plaque,Preliminary research the the role of CD105 in atherosclerosis plaque stability and discuss the effect of rosuvastain intervention in plaque stability.methods 1.Establishing animal models of atheroscletic Prepare 36 healthy male SD rats which weight between 160g-200 g, then divided into control group(n =12) and experimental group(n=24) randomly after one week adaptive feed.Four weeks after the high-fat diet, the experimental group was randomly divided into two subgroups(12 rats for each subgroup)model group and drug intervention group 2.Rats in the drug intervention group were randomize receive gavage with 5mg/kg.d rosuvastain dissolved in 1ml distilled water,the others in control group and model cantrol were randomize to receive gavage 10ml/day distilled water. 3.At the end of 8 weeks,all SD rats were killed by an overdose of intravenous Chloral hydrate sadium.The rats thoracic aortas were quickly move to make HE staining。The morphological charateristics of plaque were observed under light microscopy.In order to unequivocal the expression of CD105 in the plaque,the monoclonal antibodies against ofCD105 were applied for immunohistochemical staining of the rat thoracic aortas.In order to make clear expression of CD105 protein in plaques local, RT-PCR were used to detect expression level of CD105 m RNA in the atherosclerotic plaque.results 1.the SD rats grew well generally.All the 18 SD rats were used in result analysis,there are 12 SD rats in model group,12 SD rats in drug intervention group,12 SD rats in control gruoup. 2.Analyses of Histological and Morphology The result of HE staining of the rats thoracic aorta:the rats thoracic aorta wall on the control group was normal,smooth intima, endothelial cells complete ande order,the elastic layer was clear, smooth muscle cells in the membrane were order and form rule, elastic fiber is normal, not seen obvious plaque formation;the model group vascular cavity was smaller, intima obviously thickening, a lot of smooth muscle cells hyperplasia under the intima, lipid plaque, a lot of foam cells to gathered, and the typical unstable plaques, there mian show a thin fibrous cap,larger lipid core, a lot of inflammatory cells infiltration,a lot of scattered and clusters of small blood vessels in the unstable plaques, the rat thoracic aorta in the drug intevention gruop can find typical stable plaques,the plaques main show thicker fibrous cap, fibrous cap is complete, lipid core is small, a few of smooth muscle cells in the lipid core, a few of inflammatory cells infiltration and the tiny blood vessels. 3.The results of Immunohistochemincal In all thoracic aorta, there were CD105 expression, the CD105 have high expression in the unstable plaques in model control group, while it ismain expression in the cell membrane,and the CD105 expression in the drug intervention group stable plaques less than the model control group. the CD105 were expression less and scttered in the control group,on the basis of result of quantitative analysis,we have observed that:the expression value of CD105 in model group have significantly diffrents compared with the control gruoup(P<0.05),the expression value of CD105 in model group have significantly diffrents compared with the drug intevention gruoup(P<0.05),the expression value of CD105 expression in drug intevention group have significantly diffrents compared with the control gruoup(P<0.05)。 4.the result of fluorescence quantitative PCR The expression of CD105-m RNA were observed in each group,expression value of CD105-m RNA in each group have a significantly differents(P<0.05),the end of 8 weeks,the value of CD105-m RNA in model group have a significantly differents compared with control group(P<0.05),CD105-m RNA have a high expression in model control group than drug intevention group,the end of 8 weeks,the CD105-m RNA have a significantly differents in drug intevention compared with control group(P<0.05),the drug intevention grup have a high expression of CD105-m RNA than control group.Conclusion 1.the value of CD105 have significantly increased in the local of unstable plaque, 2.in the palques,the rosuvatation can remarkabla inhibit the expression of CD105 to play a role in plaques stability. |
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