| Background and Aim: Brain aging covers the main character of nervous system aging, and has reported to a main contributor to the pathogenesis of neurodegenerative diseases. Its detailed mechanism,however, is still unclear. Recent evidence has demonstrated that an age-related increase in the levels of oxidative stress is closely related to the brain aging and neurodegenerative diseases. Therefore, this study will explore the mechanism of oxidative stress for brain aging and its role in related diseases using proteomics and other experimental techniques.Methods: After successfully constructed aging model of rats, several analyses including deep sequencing, proteomics and biochemical assays were used to explore the relationship of oxidative stress with brain aging.Results:(1) Based on the results from the analyses of deep sequencing and bioinformatics, a series of oxidative stress-associated mi RNAs were displayed in different aged rats. Their expression seemed to be an age-related nonlinear model, and most of them showed an increased expression in the adult and a relative decreased expression in aged group. Especially, the peak expression of 13 mi RNAs were in adult rats, and mi R-653-3p only expressed in adult group.(2) The results from oxidative stress assays in rats serum illustrated that the level of MDA in the adult rats was the highest, although the highest concentration of GSH was observed in the young and the value of T-AOC in the old wasobviously higher than that of the other groups.(3) Further results from proteomic analysis demonstrated that 62 proteins were sensitive to the oxidative modification in different rat brains. More than half of these proteins were varied with ages. Among them, 8 proteins were uniquely identified in the young, and 20 proteins were only identified in the old group. Intriguingly, most of the 20 proteins were functioned in nervous system.Conclusion: In the aging process, a serial of oxidative stress-related mi RNAs were differentially expressed associated with age. Consistent with this, many proteins susceptible to redox modification were also showed age-related alternations. Moreover, most of oxidative modified proteins in the age group were the functional proteins of the nervous system. |
