Gender-related Clinical Characteristics And Sensitive Gene Expression Analysis In Patients With Differentiated Thyroid Cancers

Gender-related clinical characteristics in patients with differentiated thyroid cancersObjective To analyze the differences of clinical characteristics in male/female patients with differentiated thyroid cancers(DTC).Methods The cohort of this retrospective study consisted of patients with DTC who underwent surgery at a single hospitalfrom 2005 to 2014.Results (1) Females are more common in patients with thyroid cancer. (2)Whether in males or in females,serum TSH was higher in DTC than in benign, Mean ages were younger in DTC than in benign and nodules sizes were smaller in DTC than in benigen.(3)The prevalences of DTC were significantly higher in patients with a single nodule than patients with multinodulars in male patients(48.17% vs.41.71%,P<0.05), however no signifcant difference was found in female patients (45.78% vs46.24%,P>0.05).(4) The prevelance of DTC in male patients with overweight or obese were higher than patients with nomal weight,were (54.04% vs.36.36%,p<0.05),and (43.25% vs.36.36% p>0.05) respectively.However there were no significant in female patients with DTC. (5) Male had significantly higher rates of Ⅲ/Ⅳ TNM Stage, Lymph node metastasis,were (22.2%,vs 14.6%, P<0.01)、 (31.6%,vs 24.2%, P<0.01)respectively.and no significient differernce can be seen in extrathyroidal invasion (6) The results of Binary logistic regression analysis showed BMI was the independent risk factor in male DTC,and the number of the nodules has no significant relationship with male patientss. However BMI has no significant relationship with prevelance of DTC in female patients, the number of the nodules has significant relationship with prevelance of DTC in female patients.In conclusion There is significant gender-related differenced clinical characteristics in the patients with DTC. Females are more common in patients with thyroid cancer. Compared with males, females have a better prognosis.Study of screening sensitive gene in patitents with papillary thyroid cancerObjective:To screen sensitive gene different expression in patients with papillary thyroid cancer.Methods:RNA was extracted from paraffin-embedded tissues derived from PTC (n=7) and nodular goieter (NG) used as controls (n=7). AffymetrixHTA2.0 microarray assays was used to examine the different genes.Real-time quantitative PCR (RT-PCR) was employed to verify the significant differences of genes. Coding-noncoding gene network was constructed by a number of prediction softwares.Results:Compared with the controls,the mRNA and LncRNA expression profiles are significantly differentially expressed in the papillary thyroid cancers tissues(foldchange≥2.0,P≤0.05).An average of 12 mRNA were significantly up-regulated and 77 mRNA were significantly down-regulated.And an average of 15 LncRNA were significantly up-regulated and 79 LncRNA were significantly down-regulated.MT1G,MT1F,MT1H were verified by Real-time quantitative PCR(RT-PCR),and the RT-PCR results were consistent with the microarray data. According to the pearsons of mRNA and LncRNA,we construct the coding-noncoding network,we found LncRNA:n382996, n342483, n409114 had a close relation with mRNA:MT1G, MT1H, MT1F.Conclusions:MT1G, MT1F, MT1H were significantly down-regulated. MT1G, MT1F, MT1H may be the inhibiting factors of the papillary thyroid cancer.