Expression And Clinicopathological Correlative Analysis Of Oct4 And Wnt/β-catenin In Colorectal Cancer

Objective:Colorectal cancer(CRC) is one of the most common malignant tumors with increasing incidence in the world and nearly 600000 cases of new cases were increased per year. Therefore, how to detecte and diagnoseearly to reduce the morbidity and mortality of CRC, has become a topic of tumour researches. The changes in oncological molecular biology have been confirmed as a new neo-index to distinguish metastasis and predict prognosis. So it would be great significance to search for better markers for the early diagnosis, prediction of recurrence and metastasis of CRC.Up to now, few studies on the significance of OCT4, Wnt1a,β-Cateninin expression in CRC were found. Works on the possible relationship between the expressions of them especially on metastasis of CRC have not been reported in the literature.To further explore the mechanism of the development and metastasis in the colorectal cancer and to find sensitive indicators, the expressions of OCT4, Wnt1a,β-Cateninin normal colorectal mucosa, colorectal adenoma and CRC was studied with SP immunohistochemical staining tecnique.Methods:The expressions of OCT4, Wnt1a,β-Cateninin were studied in 95 cases of primary CRC,23 cases of colorectal adenoma and 12 cases of normal colorectal mucosa with SP two step methods. The relationship between the expressions of these three markers in CRC and their clinical pathological significance and the prognosis of CRC were also analyzed.Results:1 The positive rates of Oct4, Wnt1a were 58.9%(56/95),42.1%(40/95) in CRC, while 34.8%(8/23) and 17.4%(4/23) in colorectal adenoma and 16.7%(2/12) and 8.3%(1/12) in normal colorectal mucosa, respectively. Statistical analysis indicated that the Oct4, Wntla positive rates in the colorectal carcinoma differed significantly from that in the colorectal adenoma and normal colorectal mucosa (P<0.01).2 The expression of β-Catenin was at membrane in the normal colorectal tissue, while at cytoplasm or nucleus in the colorectal adenoma and colorectal carcinoma. It was significantly different between the two groups(P<0.01).3 The expression of Wntla and Oct4 were significantly correlated with TNM stage and lymphatic invasion(P<0.05). The expression of Oct4 was also significantly correlated with tumor differentiation(P<0.05). The expression of Wntla was also significantly correlated with distant metastasis(P<0.05). The abnormal expression of β-Catenin was significantly correlated with TNM stage and lymphatic invasion(P<0.05).4 There was a high positive correlation between the abnormal expression of β-Catenin, Wnt1a and Oct4 expression in colorectal carcinoma, respectively.5 There was a high positive correlation between the abnormal expression of β-Catenin, Wnt1a and Oct4 expression and the prognosis of CRC.Conclusion:1 The expression of OCT4, Wnt1a,β-Cateninin to some extent was correlated to cancerization of colorectal carcinoma cells, TNM stage, lymph node metastasis, distant metastasis and so on, and might play a role to predict the metastasis of CRC latently.2 There was a high positive correlation between the abnormal expression of β-Catenin, Wntla and Oct4 expression and the prognosis of CRC.3 There was a high positive correlation between the abnormal expression of β-Catenin, Wnt1a and Oct4 expression in colorectal carcinoma, respectively. They may play roles to promote proliferation and metastasis of CRC cells.