Studies On The Extraction Process And Quality Control For Jiejiuling

Object:The chemical composition of Cucumis m elo L is composed of cucurbitacin, it contains the cucurbitacinB、 cucurbitacinE, the cucurbitacinB、cucurbitacinE is the main active substance.Clinically used for drinking, chung spit, jaundice, hepatitis, the treatment of tumor.Therefore, this topic to optimize the extraction process of melon pedicleCucumis melo L. and establish its quality control method.Methods:his experiment preparation using L9 (34) orthogonal design, the ethanol reflux method to extract the medicinal materials, to extract rate to evaluate the extraction effect of index, the optimization, the best preparation conditions. Quality control of the TLC of spirit in the drinking gourd,cucurbitacin B, cucurbitacin E and Cucumis melo L, the original medicinal materials for the qualitative identification; A t the same time to block cucurbitacin B, cucurbitacin E index, HPLC method to determine its content to the overall control of the drug quality.Result:The optimum extraction process was 80% ethanol reflux extraction, extracted 2 times, each time 60 min, and solid-liquid ratio of 8 ml/g;Extract the reduced pressure concentration, removing impurity, drying dry paste made from extract;Thin ayer identification effect is clear, exclusi ve;This experiment has established the spirit in the drinking gourd cucurbitacin B, cucurbitacin E identification and content determination method, survey of methodology meets the requirements.Conclusion:to optimize the extraction technology is reasonable stable and reliable;Quality control methods are accurate and reproducible, simple, provide clinical curative effect, the medicine of the quality is stable and controllable, laid the foundation of pharmaceutical experiment.