Expression Of HIF-1α And PTEN In Human Osteosarcoma And Its Clinical Significance

objective:This paper aims to test the expression of HIF-1α and PTEN in clinical samples of osteosarcoma and osteochondroma through immunohistochemical techniques, explore the possible relationship between HIF-1α and PTEN, and osteosarcoma, investigate the relationship between HIF-1 alpha and PTEN, and clinicopathologic features of osteosarcoma,and provide a new theoretic framework for diagnosis and treatment through correlation analysis of expression of HIF-1α and PTEN.Methods: This paper randomly collects the clinical cases of osteosarcoma and osteochondroma and classifies these samples according to different pathological features, detects the expression of HIF-1α and PTEN in each case with the immunohistochemical method, analyzes the data with SPSS 18.0 based on the results of immunohistochemistry and various pathological features to discuss whether there is any correlation between the expression of HIF-1α and PTEN, and the following elements: clinicopathological characteristics such as osteosarcoma pathological grade and stage, tumor differentiation, age, gender, tumor size, and the prognosis of patient, and the prognosis of the patients.Result: 1. The expression levels of HIF-1α in osteosarcoma is significantly higher than that in osteochondroma, and the difference between them is statistically significant(P < 0.05); the expression levels of PTEN in osteosarcoma is significantly lower than that in osteochondroma, and the difference between them is statistically significant(P < 0.05); and the expression of HIF-1α and PTEN in osteosarcoma is negatively correlated(P<0.05, r =-0.449) according to the correlation test.2. In osteosarcoma, the HIF-1α positive rate in IIb-III patients is significantly higher than I-IIa patients in the expression of HIF-1α in Enneking staging, and the difference is significant(p<0.05); in different pathological grade, the positive rate of HIF-1α in patients with with low differentiation is significantly higher than that with medium and high differentiation, and the difference is significant(p<0.05); in the presence or absence of lung metastasis, the positive rate of HIF-1α in patients with lung metastasis is significantly higher than in patients without pulmonary metastasis, and the difference is significant(p<0.05); in the prognosis of patients, the positive rate of HIF-1α in patients with relapse is significantly higher than that of patients without relapse, and the difference is significant(p <0.05); in some other pathological features, the HIF-1α expression has no correlation with the patient’s age, gender, and tumor size, and the difference is not significant(p> 0.05).3. In osteosarcoma, the positive rate of PTEN in patients with pulmonary metastasis is significantly lower than that in patients without pulmonary metastasis, and the difference is significant(p <0.05); in the prognosis of patients, the positive rate of PTEN expression is significantly lower in patients with relapse than patients without relapse, and the difference is significant(p <0.05); in some other pathological features, the PTEN expression has no correlation with the Enneking staging and grading, patient’s age, gender and tumor size, and the difference is significant(p > 0.05).Conclusion: Through the pathological detection of the expression of HIF-1α and PTEN in osteosarcoma clinical cases, this paper reveals a negative correlation between HIF-1α and PTEN in osteosarcoma, suggests that these two molecules may be regarded as potential molecular markers for detecting the degradation level of osteosarcoma patients, and may provide a favorable theoretical basis for targeted therapy on patients over HIF-1α and PTEN.