Effect Of MiR-103a-3p On Proliferation, Migration And Invasion Of PANC-1 Cell Lines

Objective: Pancreatic cancer is one of ganstrointestinal tumor with hign biological properties of invansion and metastasis, and mi RNA was closely related to the processes and invansion and metastasis of pancreatic cancer development.Our Preliminary experiments by q RT-PCR detection of mi R-103a-3p expression in pancreatic cancer cell lines significantly increased, and through transfection lentiviral of mi R-103a-3p-inhibition, thereby obtaining a stable transfected cell line PANC-1. This study aims to understand the impact of the PANC-1 pancreatic cancer cell’s proliferation and migration and invasion abilities which the mi R-103a-3p did to on cellular level and provide experimental and theoretical basis for the in vivo study of genetic mechanisms and the next target.Methods: 1. Our Preliminary experiments is to select the PANC-1 cell lines as our experimental subjects and take 1ul, 5ul, 10 ul, 20 u of The mi R-103a-3p lentiviral vectors werel and empty viral vectors to transfected the PANC-1 cell lines.2.Use the fluorescence microscopy to observe the transfection efficiency, then test its expression by q RT-PCR, and selected the set of mi R-103a-3p minimum expression of lentivirus transfected PANC-1 cell lines and empty viral transfection of PANC-1 cell lines.3.Divided it into three groups: 1) inhibition of lentiviral mi R-103a-3p expression vectors infected PANC-1 group(control-PANC-1); 2) empty viral vector-infected cell line PANC-1(NC-PANC-1); 3) the group of normal pancreatic cancer cells(PANC-1).4. MTT assay to detect the proliferation ability of the three cell lines;5. wound healing test to detect the migration of three groups of cell lines;6. Transwell chamber assay to detect the invasion ability of three groups of cell lines.Results: 1) The transfection efficiency was observed After we transfected the mi R-103a-3p-inhibitor lentivirus and empty viral vector into PANC-1 cell line, then we get the mi R-103a-3p low expression of PANC-1 cell lines.2)The test results of the MTT assay shows: the number of the control-PANC-1 group of PANC-1 cells was significantly reduced compared with the PANC-1 group and the NC-PANC-1,(P <0.05), and with the incubation time, the control-PANC-1 group’s growth rate of the number of cells was waning than the PANC-1 group and the NC-PANC-1 group.3) The results of the wound healing shows: reduced the amount of the expression of mi R-103a-3p,the healing abilities of the control-PANC-1 group was significantly lower than the NC-PANC-1 groups and the PANC-1groups.4) The results of the Transwell chamber shows: reduced the amount of the expression of mi R-103a-3p,the invasion ability of the control-PANC-1 group was significantly decreased than the NC-PANC-1 groups and the PANC-1groups.Conclusion: After we reduced the amount of the expression of mi R-103a-3p,it can inhibit the proliferation、migration and invasion of PANC-1 pancreatic cancer cell, so we indicat that the mi R-103a-3p’s expression in pancreatic cancer cells can promote the proliferation、migration and invasion of PANC-1 pancreatic cancer cells.