MiR-103a-3p Suppresses Cell Proliferation And Invasion By Targeting Tumor Protein D52 In Prostate Cancer

Objective: Prostate cancer is one of the most common cancers in men.The currently available treatments have improved patient survival with local prostate cancer,but have also shown serious side effects.In many countries,the incidence of prostate cancer increases with age.Therefore,early diagnosis is an important step for clinical evaluation of patients and selection of effective treatment.Studies have shown that noncoding genes are also involved in tumorigenesis and development,especially microRNAs(miRNAs),which regulate the biological functions of various cells in cancer.Therefore,the study of the function and molecular mechanism of miRNAs will bring new understanding to the biological process of tumors.Although abnormal regulation of microRNA-103a-3p(miR-103a-3p)has been reported in a variety of human cancers,its expression in prostate cancer remains unknown.The purpose of this study was to investigate the role and potential mechanisms of miR-103a-3p in prostate cancer.Methods: To investigate the role of miR-103a-3p in prostate cancer,we performed clone formation,wound healing,invasion,proliferation,and apoptosis experiments.The target gene prediction software TargetScan was used to predict the potential targets of miR-103a-3p.In addition,the target gene expression of miR-103a-3p was detected by western blot and immunofluorescence.Finally,we verified the binding of miR-103a-3p to TPD52 using a dual-luciferase reporter gene experiment.Results: The results show that overexpression of miR-103a-3p in prostate cancer cells can significantly inhibit the proliferation and invasion of prostate cancer cells,and promote apoptosis.miR-103a-3p is capable of inhibiting its expression by directly binding to the 3'UTR of the tumor protein D52(TPD52).In addition,we also found that overexpression of TPD52 significantly reduced the effect of miR-103a-3p on prostate cancer cell function.The results of in vivo tumor formation experiments in nude mice showed that miR-103a-3p inhibited the growth of prostate cancer cells in vivo.Conclusion: Our study demonstrates that miR-103a-3p directly targets TPD52 and inhibits the proliferation and invasion of prostate cancer.This finding helps clarify the role of miR-103a-3p-TPD52 axis in prostate cancer,and provides a certain theoretical basis for the screening and treatment of tumor markers for prostate cancer.