Targeted Thrombolysis For Deep Venous Thrombosis

【Objectives】After the synthesis of carboxylated superparamagnetic Fe3O4 nanoparticles(MNP),urokinase(UK)was immobilized to the MNP. An arteriovenous shunt thrombosis model was established to evaluate the efficacy and safety of the cross-linked drug.【Methods】Carboxylated superparamagnetic magnetic nanoparticles(MNP) coated with N-oleoylsarcosine(NOSARK) was synthesized and immobilized to UK by using EDC/(N-Hydroxysulfosuccinimide) Sulfo-NHS as a cross-linking agent to promote formation of amide bound between activated carboxyl group of MNP and amino groups of urokinase. the efficacy and safety of the cross-linked drug was evaluated in an arteriovenous shunt thrombosis model by measuring the wet weight of thrombus, the concentration of plasma fibrinogen(Fbg) and the bleeding time.【Results】1. The result of characterization:MNP shows good stability, dispersion, biocompatibility and super paramagnetic.2. Thrombolysis efficiency: The thrombolysis efficiency of group MNP-UK/M was 3.6 times of group UK and 1.9 times of group UK-MNP.3. The concentration of plasma fibrinogen:No significant decrease of Fbg was observed after treatment with MNP-UK/M(P<0.05),4.Bleeding time:The bleeding time was not significantly prolonged after treatment with MNP-UK/M(P<0.05).【Conclusions】Compared with the traditional thrombolytic therapy, thrombolysis with MNP-UK/M shows higher efficiency and better security。...