| Background Breast cancer is a kind of malignant diseases which is seriously harmful to women’s health. Metastasis of breast cancer occurs early, cancer cells can spread along the catheter, fascial space or lymphatic into vein, and cancer cells can also enter blood circulation by micro vessels and cause distant metastasis. Currently, HPSE (Heparanase, HPSE) is the only kind of founded incision enzyme that is able to degrade extracellular matrix and basement membrane heparan sulfate enzyme. The latest studies of HPSE in breast cancer area focus on two parts, one is HPSE’s high expression in cancer tissues, another is it promoting cells invading and tissue angiogenesis. Few studies deals with the expression of HPSE in serum, the relation of the protein and clinicopathological features in cancer tissues, and whether HPSE can promote cancer lymphangiogenesis.Purpose To study the expression of HPSE in serum and tumor tissues in breast carcinoma and its relationship with clinical pathological characteristic, LMVD marked by D2-40 in breast tumor tissues.Method Cases are selected from Department of general surgery of First Affiliated Hospital of Xinxiang Medical University from February 2013 to May 2014. The treatment group is 50 patients with breast cancer, and control group is 20 healthy women. Firstly, test the serum HPSE level of 30 patients randomly selected in treatment group and 20 healthy women in control group by enzyme-linked immunosorbent assay (ELISA). Then, use immunohistochemical method to test the expression of HPSE in the carcinoma tissues of the 50 breast cancer patients in treatment group, and also test the expression of HPSE in the corresponding non-tumor normal breast tissues. Last, The LMVD is signed by D2-40, and test the expression of D2-40 both in the breast cancer tissue and the corresponding non-tumor normal breast tissues.Results 1) The serum HPSE level was significantly higher in patients with breast cancer (112.69±28.67u/ml) than in the control group (81.65±15.15 u/ml) (P<0.01);2)The HPSE protein was mainly expressed in the cytoplasm or membrane of cancer cells.The positive expression rate of HPSE was (34/50,68%) in breast cancer tissue,which was significantly higher than that in adjacent normal tissue (0/50,0%)(P<0.01).HPSE expression in breast cancer tissue was closely related with tumor size,TNM stages,histological grade and axillary lymph nodes metastasis(P<0.05),and not related with age (P> 0.05).3) The lymphatic microvessels signed by D2-40 mainly located around the center of breast cancer tissue with irregular shape. There are less lymphatic microvessels in the center of breast cancer tissue, and most of them are narrow like streak. The positive rate of D2-40 was (44/50,88%) in breast cancer tissue, which was significantly higher than that in adjacent normal tissue (15/50,30%)(P<0.01).The LMVD around breast cancer tissue was (5.30±3.10), LMVD of central breast cancer tissue was (1.86±0.52),and adjacent normal breast tissues LMVD was (1.82±0.35), The LMVD in the area around cancer was significantly higher than the central area and adjacent normal breast tissue (P<0.05).4) Along with the increasing of HPSE expression, the LMVD in the area around cancer tissue also increasing gradually. The expression of HPSE and the LMVD in breast cancer tissue show a positive correlation(r=0.67,P<0.01).Conclusion 1) The levels of HPSE in serum of patients with breast cancer are significantly higher than healthy women, which indicate that detection of serum HPSE is helpful for screening and diagnosis of breast cancer before operation;2) The protein qualitative results showed that the expression of HPSE in breast cancer tissue is high,and closely related with tumor size,TNM stages,histological grade and axillary lymph nodes metastasis.The result shows that HPSE promotes the invasion and metastasis of breast cancer,the detection of HPSE can be used to evaluate the biological characteristics of the tumor.3) HPSE may promote lymphangiogenesis of breast cancer, The research provides a theoretical basis for anti lymphangiogenesis drug selection, design and clinical application of breast cancer. |
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