Umbilical Cord Mesenchymal Stem Cells Joint Nerve Conduits Between The Research For The Treatment Of Femoral Nerve Defect In Rats

Objective:To investigate the umbilical cord mesenchymal stem cells (HUMSCs) applies acellular nerve catheter. Repair treatment 8mm rat femoral nerve defects, for HUMSCs repair of peripheral nerve injury clinical applications provide an experimental basis. Methods:The tissue blocks and trypsin digestion were isolated from human umbilical cord, purification and subculture HUMSCs, inverted phase contrast microscope morphology and growth HUMSCs, production 3rd generation HUMSCs growth curve, flow cytometry analysis and induction into neurons differentiation, parallel immunofluorescence staining. Secondly, the use of chemical extraction method to obtain acellular nerve. A total of 60 SD rats were randomly divided into three groups, each 20. Group A:8 mm femoral nerve cut mouth and then suture; group B:HUMSCs planted in nerve repair conduit 8 mm femoral nerve defects; Group C:nerve conduit+saline. After eight weeks, the general behavior observed (BBB rating), electrophysiological testing, retrograde tracing observation and counting, immunohistochemistry. Results:The use of the above two methods can be obtained HUMSCs. Inverted phase contrast microscope adherent cells were grown for fibroblast-like morphology. But found that cell activity by growth curve tissue explant culture better. Streaming identify the expression CD29, CD44, CD 105, did not express CD34, CD45. After induction, Nestin, GFAP and NF-M expression was positive.8 weeks after implantation cell test results show, A, group B and group C BBB score, electrophysiological and length after neurofilament NF-200 staining were significantly different (P<0.05), but the retrograde tracer mark, before injury in experimental animals in each group and the contralateral side of the spinal cord corresponding to the number of red dyed horn neurons than were no significant differences (P> 0.05). Conclusions:1.human umbilical cord contains mesenchymal stem cells, tissue blocks for primary culture method is superior to the cold trypsin digestion; and the ability to HUMSCs neurons induced.2.HUMSCs combined acellular nerve can effectively promote the repair of rat femoral nerve injury.