The Study Of The Role Of Alveoli Formation Gene-P311 In Hyeroxia Lung Injury

Part One The Expression and Significance of AlveoliForming Gene-P311 in Lung Tissue of Mice about HighOxygen Causing bronchopulmonary dysplasiaObjective: To study the expression and significance of alveoli formation gene P311 in lung tissue of mic about high oxygen causing bronchopulmonary dysplasia. Methods: 64 newborn(4 days age) of KM mice were divided into air group(the control group) and high oxygen group(the experimental group), each group of 32, respectively by the mother feeding, every female mouse with 8~10 newborn mice, air group in the normal pressure feeding, high oxygen groups in the same room continuous inhaled oxygen concentration in airtight oxygen chamber > 90% of the medical oxygen, air group and high oxygen group taking the lung tissue after respectively 1,3,7and 14 days(8 in each group), then observ the lung tissue pathological changes and authenticate whether the model building successful by hematoxylin-eosin staining; Further immune-histochemical staining and real-time quantitative polymerase chain reaction methods to detect P311 change and the distribution of gene expression; By enzyme linked immune-sorbent assay method to detect P311 protein. Results: The lung tissue pathology shows that high oxygen group compared with air control group, as the oxygen inhaling high concentrations with the extension of time, the lung tissue express as alveolar number decrease, volume increase, pulmonary structure simplification and microvascular dysplasia, confirm successful building high oxygen caused bronchial pulmonary dysplasia animal model. Immuno-histochemical staining of lung tissue, P311 are mainly distributed in the alveolar walls and alveolar septum growing point in the lung tissue,which in the air were weakly positive reaction in the lung tissue, with strong positive reactions in the lung tissue of mice in the high oxygen group. Newborn mice P311 gene expression and protein content in 7 day reach to peak, compared with the air control group, high oxygen group P311 gene expression and protein content increased obviously(P<0.05); Conclusion: Inhaled high concentration of oxygen for a long time can cause alveolar structure disorders in mice, alveolar and pulmonary microvascular dysplasia,which prove the model building successful; P311 gene and protein were higher in the high oxygen group, that may indicate P311 may relate with the alveolar. At the same time, that explain P311 may be a regulatory factor of lung injury repair of hyperoxia induced bronchopulmonary dysplasia.Part Two The Study of the Role of Alveoli Formation Gene-P311 in High Oxygen Causing Lung Injury of MiceObjective: To understand the role of alveoli formation gene P311 exposed to high oxygen in lung tissue injury of mice. Methods: 64 adult(60 days age) KM mice were divided into air group and high oxygeon group, air group feeding in the ordinary pressure atmospheric, high oxygen group exposed to the indoor closeness oxygen chamber, continuous inhaled oxygen concentration>90% medical oxygen, taking the lung tissue in the 1,3,7 and 14 days later, then by hematoxylin-eosin staining to observe the lung tissue pathological changes and authenticate whether model building successful;Further by Immuno histochemical staining and real time quantitative polymerase chain reaction methods to detect P311 distribution change and the gene expression situation; By enzyme linked immune-sorbent assay method to detect changes in P311 protein;Results: The lung tissue pathology change suggests that adult mice with high oxygen group inhaling high concentrations of oxygen time extension, lung tissue pathological changes is more serious than the air group; Immuno-histochemical staining of lung tissue, P311 are mainly distributed in the alveolar walls and alveolar septum growing point in the lung tissue,which in the air group had weakly positive reaction in the lung tissue, but had strongly positive reactions in the lung tissue of mice in the high oxygen group; Adult mice with high oxygen group P311 gene expression and protein content increased obviously in the air group(P<0.05); Conclusion: With inhaled high concentration of oxygen for a long time can cause lung injury more severity in mice, we successfully make a copy hyeroxia lung injury animal model.P311 gene and protein expression are higher in the high oxygen group, that even more explain P311 may be a regulatory factor of lung injury repair of hyperoxia induced bronchopulmonary dysplasia.