Study On The Effect Of Genistein And Adriamycin On The Proliferation Of Human Ovarian Cell A2780 And Growth Of Tumor Bearing Nude Mice

Investigating the effect of Genistein combined with adriamycin to cell proliferation of A2780 in Ovarian cancer and tumor growth of bearing nude mice.MethodsMaking A2780 in Ovarian cancer into suspension, Inoculating to subcutaneous of the right posterior leg heel of the SD stain nude mice, the model of ovarian cancer nude mice was accessed. Dividing into 4 treatment groups: NS group( nude mice intraperitoneal injection with 0.2ml NS), Genistein group(nude mice intraperitoneal injection with GEN30mg/kg), Adriamycin group(nude mice intraperitoneal injection with ADR20mg/m2),Genistein combined with Adriamycin group(nude mice intraperitoneal injection with GEN30mg/kg and ADR 20mg/m2).4weeks later, executing nude mice. Picking off and weighing the whole tumor tissue, calculating the tumor control rate. Testing the expression of c-myc in the tumor cells by immunohistochemistry, testing the expression quantity of c-myc by Western blot.(Methy thiazoly1 tetrazolium, MTT) testing the proliferation of A2780 cell, cell adhesion test and transwell test detected the invasiveness of cell. FCM tested the cell cycle and apoptosis.ResultsAnalysing tumor control rate,comparing with the NS group, Genistein combined with Adriamycin group, Adriamycin group, Genistein group, control rate were respectively( 44.81±5.74) %,( 38.45±4.25) % 、(32.26±2.21)%,Difference had statistical significance(P<0.05)。IMH results: Genistein combined with Adriamycin group, Adriamycin group, Genistein group all expressed c-myc, Western Blot revealed that comparing to NS Group,the expression of c-myc had distinct up-regulation(P<0.05)in Genistein combined with Adriamycin group, Adriamycin group and Genistein group.MTT revealed NS group,Genistein group, Adriamycin group, Genistein combined with Adriamycin group, cell activity respectively(100.0±0.0)%,(56.4±4.25)%、(59.26±2.21)%、(40.33±1.23)%。cell adhesion test revealed that GEN combined g with ADR group 、ADR group、GEN group could reduce the adhesion of A2780 cell(P<0.05),moreover no effect on LEC1 Cell。Cell migration test detected that the cell migration rate of GEN combined with ADR group、ADR group、GEN group,cell migration rate significantly lower than the NS group(P<0.05)。FCM test revealed that the cell population in G0/G1 stage significantly increase and in S stage reduced in GEN combined with ADR group、ADR group、GEN group(P<0.05),apoptosis rate in GEN combined with ADR group、ADR group、GEN group apparently higher than NS group(P<0.05),and no promoting apoptosis effect on LEC1 cell.ConclusionThe genistein combined with adriamycin in certain doses could inhibit the proliferation and invasiveness of ovarian carcinoma, and induce its apoptosis. The mechanism may be the decreased expression of c-myc by genistein combined with adriamycin to affect the proliferation and invasiveness of cell.