Study On Preparation And Determination Of Anti-ischemia Effective Components Groups Of Radix Ginseng And Cortex Moutan

Objective: To optimize the extraction process of the effective components groups, including total ginsenosides, paeonol and total glycosides, from Radix Ginseng and Cortex Moutan, and to set up an HPLC method for determination of various effective components in the components groups.Methods:1. Using ginsenosides Rg1, Re, Rb1, Rd as the indexes to determine contents of the 13 batches of Radix Ginseng by HPLC method; and using paeoniflorin and paeonol as the indexes to determine contents of the 12 batches of Cortex Moutan by HPLC method. Experiment materials were chosen by the determination results.2. Water and 70% alcohol as extraction solvents were used to compare the ginsenosides extraction effects.3. With ginsenosides Rg1, Re, Rb1 as the indexes, 4 main extraction influence factors, such as alcohol concentration, the amount of alcohol, extraction time, heating time for each extaction were studied, and 3 levels for each factor were observed to optimize extraction process by orthogonal experiments.4. Paeonol in Cortex Moutan was distilled, then paeoniflorin in the distillation residue was extracted by alcohol.5. Extracts above were purified respectively with macroporous adsorption resin to refine the total ginsenosides of Ginseng and glycosides of Cortex Moutan. The contents of ginsenosides Rg1, Re, Rb1, Rd in purified total ginsenosides, paeonol and paeoniflorin in the extracts of Cortex Moutan were determined by HPLC respectively.6. The total ginsenosides, paeonol and total glucosides of Cortex Moutan were prepared, and mixed as the anti-ischemia effective components group.7. HPLC was used to determine of certain effective components in the components group simultaneously. The methodology experiments were carried out.Results: 1. The determination results of the 13 batches of Radix Ginseng all couldmeet the demands of Chinese Pharmacopoeia, which stipulate that the total contentsof ginsenosides Rg1 and Re should be no less than 0.30%, ginsenoside Rb1 should beno less than 0.20%. The 12 batches Cortex Moutan all could meet the requirement ofChinese Pharmacopoeia, which stipulate that paeonol should be no less than 1.2% inCortex Moutan. No. 01 Radix Ginseng and No. 01 Cortex Moutan, which werepurchased from Yuzhou traditional Chinese medicine wholesale market, were chosenas the test materials.2. The experimental results show that using alcohol as the extraction solvent, rather than water as extraction solvent, could lead to higher yield of ginsenosides.3. The optimum extraction process of total ginsenosides was as following: Ginseng was extraceted by 6 times 70% alcohol, for 2 times, each time for 1 hour. Validation tests showed that this extract process had good repeatability.4. The transfer rate of paeonol by distillation was more than 70%.5. The yield of total ginsenosides was more than 17%, could meet the demands of Chinese Pharmacopoeia(15% ~ 25%).6. The total ginsenosides, paeonol and total glucosides of Cortex Moutancan be mixed as effective component groups.7. A simultaneous determination method was established to determine the contents of ginsenosides Rg1, Re, Rb1, Rd, paeoniflorin and paeonol in the effective component group by HPLC.Conclusion: The extraction process of anti-ishemia effective components groups from Ginseng and Cortex Moutan, was optimized. This prepare method is simple, feasible, with high yield. An HPLC method for simultaneous determination of 6 kinds of effective components in the groups above was established.The method is simple, accurate with good reproducibility and stability. It is suitable for the quality control of effective compounds group of Radix Ginseng and Cortex Moutan.