Treatment Of Mongolian Medicine Of Hosta Qingyan Fifteen Flavor Pill On Chronic Bronchitis

Chronic bronchitis (CB, referred to as chronic bronchitis) is due to a variety of factors (including infections, non-infectious factors) caused by lower respiratory tract disease and chronic obstructive pulmonary surrounding tissue (Chronic obstructive pulmonary disease, COPD). The clinical features of cough, sputum, or accompanied by wheezing and recurrent chronic process. Progress is slow, often obstructive pulmonary emphysema, and even pulmonary heart disease. Etiology of chronic bronchitis is not clear, there are still not enough to determine a number of factors. Environmental pollution, infections, smoking are considered in recent years are likely involved in the pathogenesis of CB. With the development of China’s aging population and growing environmental pollution, the incidence of CB is increasing, the quality of life is severely affected. At present, China mainly for the treatment of CB adopt comprehensive measures to prevent binding, infection control and expectorant, cough and acute exacerbation of chronic delays moved the main treatment. If breathing when associated with places antispasmodic based. At present, more specifically the lack of efficacy of the drug. Thus, the incidence of pathological CB depth study molecular mechanisms, development of effective drug has a very important significance.Mongolian Medicine Department Hosta flowers Liliaceae Hosta Hosta Hosta plantaginea (Lam.) Aschers flowers, attending CB and so on, with detoxification. Hosta Qingyan fifteen taste Mongolian medicine pill in clinical applications for chronic bronchitis, lung infections children have a good effect. With the characteristics of high-efficiency treatment. AM for lower respiratory tract local primary immune cells, widely distributed in the alveolar and airway epithelial surface, physiology and pathology of the lung and other local plays an important regulatory role.This study was to observe the Mongolian Medicine Hosta Qingyan fifth taste CB pill treatment effect on animal models, as well as in the animal model of lung tissue oxidation-antioxidant balance adjustment, and further to alveolar macrophages (AM) to study the target, explore Hosta Qingyan fifth taste Mongolian medicine pills on the possible mechanism of therapeutic action CB provide experimental evidence for the development of new drugs.The main contents are as follows:Selection of60female mice were randomly divided into normal control group, chronic bronchitis ammonia model group, Mongolian medicine Hosta Qingyan fifteen pills taste of high, medium and low-dose treatment group, compound bronchitis tablets in the treatment groups. In addition to the normal control group, the mice were YLS28A multifunction instrument of asthma induced cough timed to give ammonia inhalation, a total of30d, induction of chronic bronchitis models. Since the first31in the control group and model group of mice given normal saline, the other groups were given medicines for treatment, one times/d, a total of15d. After treatment, the mice in each group to detect lung tissue superoxide dismutase (SOD) and malondialdehyde (MDA) levels, HE staining was observed in each group of mice lung histopathology.The results showed that fifteen taste of Mongolian medicine Hosta Qingyan pill group compared with the model group, chronic bronchitis, decreased lung homogenate MDA content in lung homogenates SOD increased, the degree of change in dosage dose-effect relationships; HE staining Hosta Qingyan after fifteen taste Mongolian medicine pill to treat chronic bronchitis inflammation improved significantly. These results confirm Hosta Qingyan fifteen taste of Mongolian medicine pill has to ammonia-induced chronic bronchitis treatment, the mechanism may be Hosta Qingyan fifteen taste of Mongolian medicine pill against oxidative damage, enhance the vitality of the body’s antioxidant enzymes, regulate oxidation-resistant oxidative balance, and thus play a role in the treatment of chronic bronchitis.separation and purification AM, detected by ELISA Hosta Qingyan fifteen taste of Mongolian medicine pills on LPS induced AM cultured supernatant TNF-α levels, Western blot was used to detect the rat AM TNF-α and pERK protein expression levels, while the application ERK antagonist (PD98059) observed AM the TNF-α protein expression. The results showed that fifteen taste Mongolian medicine Hosta Qingyan pill dose-dependent reduction in LPS-stimulated cultures due to AM due to TNF-a content in the supernatant was increased; Hosta Qingyan fifteen taste of Mongolian medicine pill (100mg/L) can significantly reduce Since pERK protein expression in LPS-stimulated cells and lead to the AM TNF-a increased; application specific ERK inhibitor (PD98059) Qingyan fifteen taste of Mongolian medicine Hosta Hosta Mongolian medicine pills and pill+PD98059Qingyan fifth taste after the intervention, we found that compared with LPS stimulation group, the expression of TNF-a in rat AM significantly reduced. Hosta Qingyan infer fifteen taste Mongolian medicine pills can induce TNF-a expression AM by inhibiting LPS, blocking the AM-induced inflammation, one of the mechanisms of action may be related to Mongolian medicine pills taste Hosta Qingyan fifteen inhibition of extracellular signal transduction pathway.