Correlation Of FFA, UCP-1Level And Obesity In T2DM Patients

Objective To observe the changes of serum FFA and UCP-1in patients with type2diabetes and healthy controls in different body mass index.To analyze thecorrelationship among FFA, UCP-1, metabolic parameters and islet beta cell function andinsulin resistance index. To explore the roles of FFA and UCP-1in the development ofT2DM and obesity.Methods According to body mass index (BMI), healthy control group consisted of38persons,were divided into normal weight group (group A,13cases, BMI <24kg/m2),overweight group (group B,14cases,28kg/m2> BMI≥24kg/m2), obesity group (group C,11cases, BMI≥28kg/m2).62cases of patients with type2diabetes were set asexperimental group, which was divided into normal weight group (group D,24cases,BMI<24kg/m2), obesity group (group E,25cases,28kg/m2> BMI≥24kg/m2), overweightgroup (group F,13cases, BMI≥28kg/m2). Double antibody sandwich enzymelinkedimmunosorbent assay (ELISA method) was used to detect UCP-1, FFA level; Totalcholesterol (TC), Triglyceride (TG), high density lipoprotein cholesterol (HDL-C), lowdensity lipoprotein cholesterol (LDL-C), fasting blood glucose (FBG),fastinginsulin (FINS) and glycosylated hemoglobin(HbA1c) were detected by the biochemicalanalyzer, and the homeostatic model assessment model was used to evaluate insulinresistance index (HOMA-IR).Results (1) Variance analysis showed:①Compared with the control group, TC,LDL-C,TG, HbA1C, FPG, FINS, HOMA-IR were higher in T2DM group (P <0.01or <0.05).②Compared with group E and group D, TC, LDL-C,TG, FINS, HOMA-IRwere higher in group F (P <0.05or <0.01), and with the increase of body mass index,FINS, HOMA IR level were correspondingly increased (P <0.05or <0.01);③Amongsubgroups of two groups, LDL-C,TG in group D, TC, LDL-C,TG in group E andTC,LDL-C,TG in group F were higher compared with normal control group correspondingbody mass index (P <0.01or <0.05).④FFA levelof T2DM group was increased compared with the control group (P<0.05); Among subgroups in T2DM, FFA level ingroup E and group F were higher compared with group D (P<0.05), FFA level in thesubgroups of T2DM were up regulated compared with the normal control groupcorresponding body mass index (P <0.01or <0.05).⑤UCP-1level in the normal groupand type2diabetes mellitus group were down regulated with the increase of body massindex (normal group r=-0.1848, P <0.01; type2diabetes mellitus group r=-0.169, P <0.01); UCP-1level in group D was down regulated compared with the corresponding bodymass index group in the control group (P<0.05), UCP-1level both in overweight andobesity group were down regulated, but the difference has no statisticalsignificance(P>0.05).(2) Univariate analysis showed:①The serum FFA level was positively correlatedwith BMI, TG, TC, LDL-C, HbA1C, FINS, HOMA-IR (r=0.479,0.295,0.313,0.365,0.315,0.505,0.533, P <0.05or <0.01), and there has no statistical correlation between serum FFAand HDL-C、FPG (r=-0.042,0.249, P=0.796,0.121).②There was negative correlationbetween the level of UCP-1and BMI(r=-0.342，P <0.05), but no significant correlationbetween serum UCP-1and TG, TC, HDL-C, LDL-C, HbA1C, FPG, FINS, HOMA-IR(r=-0.273,0.012,0.203,-0.147,-0.02,-0.223,-0.044,-0.073, P>0.05).(3) Multiple linear stepwise regression analysis showed: BMI (β=0.536，P=0.025)and HOMA-IR (β=1.814，P=0.006) were independent factor of FFA, and BMI was aindependent risk factor of UCP-1(β=-0.041，P=0.031).Conclusions1. Disturbance of lipids metabolism is an important risk factor in thepathogenesis of T2DM.2. FFA may be associated with insulin resistance, so detecting itand early interfering may ameliorate insulin resistance, which may control glucose anddelay the progression.3. UCP-1level has negatively correlated with the incidence ofT2DM.