| Objective:Through DNA methylation chip detection, non blood of coronary heart disease and blood stasis differentially methylated DNA gene and from selected CTNNB1and des gene,"to measured syndrome by subtraction of Yangxin through pulse series of unrelated coronary heart disease (Qi deficiency and stagnation of phlegm, stagnation of Qi) and blood stasis syndrome subtypes of the intervention of traditional Chinese medicine and to investigate the blood stasis syndrome of coronary heart disease susceptibility gene methylation modification and expression mechanism, for TCM clinical prevention and treatment of coronary heart disease and blood stasis syndrome provide ideas and theoretical basis.Methods:Infinium were first introduced in the Human Methylation450BeadArray gene DNA methylation chip detection method (for non blood collected3cases of coronary heart disease, blood stasis group,3cases of coronary heart disease of blood stasis group and health control group3cases testing), choose the non blood coronary blood stasis CTNNB1, DES two susceptibility genes methylation changes, and compare the three groups of related clinical data;Second to non related coronary heart disease (CHD) subtype of blood stasis syndrome, add and subtract yangxin unclogged arteries party with rt-pcr technique before and after the intervention of the selected two candidate gene expression level, income, total of23cases of experimental group (coronary heart disease (CHD) and qi stagnation and blood stasis syndrome group, the coronary heart disease, phlegm turbid blood stasis group all the8cases,7cases of coronary heart disease (CHD) deficiency of blood stasis group), Chinese traditional medicine after the intervention, a total of18cases of blood sample collection (6cases of qi and blood stasis syndrome and phlegm turbid blood stasis and6cases of6patients with qi deficiency and blood stasis), through add and subtract yangxin unclogged arteries parties selected two susceptible gene expression before and after the intervention level and blood lipid, blood sugar index comparison, to explore the blood on the add and subtract yangxin unclogged arteries party sex coronary heart disease (qi deficiency, phlegm turbidity, and qi stagnation) subtype of blood stasis syndrome CTNNB1,DES two gene expression, DNA methylation modification, effect on blood lipid, research, and compare the three groups of related clinical data before and after treatment.Results:Select line between-muscle proteins Desmin (DES), serial protein (cadherin-associated, beta11,88kda CTNNB1) two susceptibility DNA1methylation gene; Blood sugar, blood fat index in non related CHD than blood stasis group, CHD group, blood stasis and the differences between the normal control group not significant (P>0.05), pairwise comparisons between groups had no statistical significance (P>0.05); But blood sugar levels in coronary heart disease (CHD) and the blood stasis syndrome of blood stasis syndrome group were significantly higher than the normal group and blood lipids TC, TG, LDL-C value in coronary heart disease, blood stasis and blood stasis group is significantly higher than normal group; HDL-C value in the normal group slightly higher than the coronary heart disease (CHD) and the blood stasis syndrome of blood stasis syndrome group.Different syndrome type of coronary artery disease in traditional Chinese medicine treatment, compared with before treatment DES gene expression were significantly decline, and there are statistically significant, including phlegm turbidity and blood stasis syndrome group(P0.01),and qi stagnation blood stasis group (P<0.05),qi deficiency blood stasis group(P<0.05); But in different syndrome types of patients after treatment, compared with before treatment CTNNB1gene expression change is not obvious, and there is no statistical significance, the phlegm turbid blood stasis, qi and blood stasis syndrome group qi deficiency and blood stasis syndrome group (P>0.05); Different syndrome types of patients after treatment, compared with before treatment the blood sugar,TC,TG,HDL, LDLC values have declined, comparison between groups have no statistical significance; Through their own matching symbols of rank and inspection, qi deficiency and blood stasis group blood sugar levels before and after the change was statistically significant(P<0.05), three sets of TC value changes before and after treatment was statistically significant(P<0.05), TG values change before and after the qi and blood stasis group was statistically significant (P<0.05), the other has no statistical significance(P>0.05).Conclusions:1Non related coronary blood stasis exists obvious dyslipidemia.2DES and CTNNB1genes of DNA methylation modification may be susceptible to risk factor for coronary heart disease (CHD) blood stasis.3Modified Yangxintongmai formula can obviously improve coronary heart disease (CHD) subtype of the lipid of blood stasis syndrome and abnormal blood sugar, have very good sugar, lipid effect.4Modified Yangxintongmai formula can for coronary heart disease (qi deficiency and qi stagnation, phlegm turbidity and blood stasis three subtypes DES gene expression had obvious regulation effect, but has no obvious CTNNB1gene expression regulation function; Further confirmed DES gene methylation modification is one of the pathogenesis of coronary heart disease, blood stasis. |
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