Yangxing Tongmai Formula For PCHD Family Of Blood Stasis SyndromeCHL1, ZEB2Research On The Effects Of Gene Methylation

Background:coronary heart disease (CHD) morbidity and mortality is on the rise in recent years, and the more and more younger. Family and genetic factors in the occurrence of early onset coronary artery disease play an important role. Research suggests premature coronary heart disease (PCHD) is a polygenic disease, and premature coronary heart disease in blood stasis is one of the most common TCM syndromes.Objective:to clarify the leading cause of early onset coronary heart disease, blood stasis, genetic risk based on DNA methylation early onset coronary artery disease in vein side of yangxin passed family of blood stasis syndrome before and after the intervention of the crowd, for the prevention of traditional Chinese medicine and promote the clinical treatment of coronary heart disease (CHD) provide new train of thought and theoretical basis of blood stasis syndrome.Methods:first USES Infinium Human Methylation450BeadArray gene DNA methylation chip detection method, choose the family early onset coronary heart disease, blood stasis CHL1, ZEB2two susceptibility genes methylation change, and compare the three groups of related clinical data; Second in Yangxing Tongmai Formula unclogged arteries before and after intervention using rt-pcr technology selected two candidate gene expression levels,60cases of total income for the experimental group (20cases of early onset coronary heart disease, blood stasis group and healthy group20cases,20cases of healthy group), traditional Chinese medicine (TCM) after the intervention were36cases of blood (blood stasis group of12cases of12groups of patients, the health, health group of12cases), through Yangxing Tongmai Formula unclogged arteries parties selected two susceptible gene expression before and after the intervention level and blood lipid, blood sugar index comparison,1to explore Yangxing Tongmai Formula unclogged arteries party home early onset coronary artery disease in the first blood stasis CHL1, ZEB2two gene expression, DNA methylation modification, effect on blood lipid, research, and compare the three groups of related clinical data before and after treatment.Results:from the family of coronary heart disease group and normal group differences of blood stasis syndrome genes selected CHL1(full name:the cell adhesion molecule with homology to L1CAM (close homolog of L1); Chinese name:cellular adhesive molecular and homology L1CAM intimate homolog (L1) and ZEB2(full name:any zinc finger E-box binding homeobox2; Chinese name:zinc finger E-box binding homeobox (2) two susceptibility genes; Glycemic index at home early onset coronary artery disease in department of blood stasis syndrome group and healthy group, and the differences between the normal control group not significant (P>0.05), pairwise comparisons between groups had no statistical significance (P>0.05); But the blood pressure value in coronary heart disease, blood stasis and subhealth group were significantly higher than the normal group and blood lipids TC, TG, LDL-C value in coronary heart disease, blood stasis and sub-health group is significantly higher than normal group. Different syndrome type of coronary artery disease in traditional Chinese medicine treatment, compared with before treatment ZEB2gene expression were significantly decline, and there are statistically significant, family blood stasis group (P=0.006<0.01), and the healthy group (P=0.021<0.05); But the three groups of patients after treatment, compared with before treatment CHL1gene expression change is not obvious, the family blood stasis group (P=0.023<0.05), and the healthy group (P=0.034<0.05), healthy group (P=0.589>0.05),; Three groups of patients after treatment, compared with before treatment the blood sugar, TC, TG, HDL, LDLC values have declined, comparison between groups have no statistical significance; Through its own matching symbols of rank and inspection, family group blood sugar levels before and after the change of blood stasis syndrome was statistically significant (P<0.05), three sets of CT value changes before and after the treatment was statistically significant (P<0.05), family group TG values before and after the change of blood stasis syndrome was statistically significant (P<0.05), other have no statistical significance (P>0.05).Conclusions:1. Yangxing Tongmai Formula for intervention, coronary heart disease, blood stasis in the treatment of remission is have obvious effect.2. Yangxing Tongmai Formula of intervention of coronary heart disease patients with blood stasis to ZEB2gene has significant effect of the regulation of DNA methylation, and for CHL1gene, no obvious change.3. ZEB2genes and early onset coronary artery disease in family correlated with blood stasis, CHL1genes and early onset coronary heart disease, blood stasis, no obvious correlation.