Activity Under The Guidance Of Schisandra Anti-tumor Effective Component Extraction, Separation And Its Anti Tumor Effect Mechanism Research

[Objective] Separation, extraction of parts of the antitumor activity of traditional Chinese medicine Schisandra, preliminary studies the anti-tumor effect in vitro and in vivo, and its mechanism of action。 Provide some experimental basis for the scientific and effective applications of the Schisandra。[Methods]1、Use the method of alcohol extraction and water precipitation to extract, separate Schisandra and get two different parts of Schisandra;2、Measure Tumor cell proliferation by MTT assay, then compared the antitumor activity of different parts of Schisandra in vitro; Mice bearing neoplasm(H22cell line)was established as the expermental tumor model, compared the different parts of the inhibition rate of the anti-tumor in vivo; according to the inhibition rate of the anti-tumor in vivo and in vitro, and the inhibition rate in vivo as the main indicators, screening of the anti-tumor active part of Schisandra initially;3、HPLC and sulfuric acid-anthrone methed identified the chemical composition of anti-tumor part of Schisandra;4、Mice bearing neoplasm(H22cell line)was established as the expermental tumor model, observed of Schisandra effective parts of the antitumor effect in vivo, and detected CTL cytotoxicity by MTT assay; mice bearing neoplasm(H22cell line)was established as the expermental tumor model, observed the Schisandra anti-tumor active part of the in vivo role of the chemotherapy drug fluorouracil synergy。[Results]1、Vitro and in vivo antitumor effect in vivo tumor inhibition rate was the main indicators, screening the Schisandra Antitumor for the water area, and HPLC and sulfuric acid-anthrone method to detect the composition of mainly Polysaccharide;2、Schisandra of different doses of polysaccharide inhibited Bearing H22mice solid tumor growth, high low-dose group inhibition rates were32.29%,54.28%,55.62%, compared with the control group has a significant (P <0.01);The Schisandra polysaccharide can also significantly increase the killing activity of CTL cells;3、The Schisandra polysaccharide combined with fluorouracil than single-agent fluorouracil, the inhibitory rate of single-agent Polysaccharide inhibition rates were55%,49%,65%. [Conclusion] The Active part of the anti-tumor of Schisandra was Polysaccharide, it has a prominent anti-tumor effect in vivo, but no significant inhibitory effect in vitro。The mechanisms may be associated with these aspects:Schisandra polysaccharide can induce apoptosis of tumor cell, and up-regulate the immune function...