Hybrid Materials Prepared And Its Application In The Molecular Detection And Cellular Activity Controlling

Hybrid material, because of its properties, was widely used. Under the biologicalmaterial participation, inorganic nano-materials and porous-membrane polymermaterials could be better applicated in biological fields. In the same time, the hybridmaterial provided a new chemical perspective in studying biological problems.Currently, CdSe quantum dots prepared operation was relatively complicated andtime-consuming, we used biological methods prepare CdSe quantum dot hybridmaterials, which was used in bilogical detection and mechanism studying; porouspolymer films were mainly application in physics and electronics fields. During thepreparation of porous membrane, DNA and PEI biological components was added in.The hybrid polymer materials could use for studying the cell activities in the controlenvironment.The thesis was divided into three parts:In the first part, CdSe quantum dots was synthesized in a easy and fast way.SeNPs were preparated under the participating of BSA and GSH. Then, it acted asintermediate to synthesize CdSe quantum dot hybrid materials. QDs were confirmedby various characterization. Its fluorescent stability had also be explored. We used theCdSe quantum dot hybrid materials to specific detecte NADPH, their linear range:0-13mM, the detection limit:250μM. In the actual serum samples, the hybrid couldalso used in detection NADPH. Finally, the CdSe quantum dot hybrid materialstoxicity was studied to e.coli cell. The results showed that the CdSe quantum dots didnot have any toxicity.In the second part, the yeast cell was used to synthesize CdSe quantum dots.CdSe quantum dot hybrid materials could be synthesized in vivo of yeast cell. After change of metabolic pathways, we identified that yeast cells synthesize CdSe quantumdots need the participation of cell mitochondrial.In the third part, DNA was used in the microemulsion droplet template method toprepare the porous membrane polymer materials. The hybrid materials porousmembrane pores was positively charged, then the negative charge GFP plasmid DNAand positively charged PEI were assemble into the pores to get a single cell array. Bythe FDA test, the “pore cell” remained activity. After re-culture the “pore cell”, itwould grow to match the pore size and do not further proliferation. All experimentssuggest that the porous membrane hybrid materials could control cell proliferationbehavior. Releasing the PEI, DNA and adding the single-stranded DNA, yeast cellscould achieve in situ transfected in the porous membranes. Re-culturing the yeastcells in the YPD(galactose acting as sugar sources), it would express green fluorescentprotein, which could be characterized by inversion confocal fluorescence microscopy.Finally, we explored the relationship between the transfection efficiency and DNAassembled layers.