Study On The Anti-fatigue And Hypoxia Tolerance Activities Of Irpex Lacteus Aqueous Extracts And Its Granule Preparation

Irpex lacteus is a species of traditional Chinese medicine, has the very highmedical value, fermentation extract Irpex lacteus has a variety of anti fatigue andimproving hypoxia tolerance activity.Irpex lacteus generally to the growth conditionsharsh requirements, Irpex lacteus wild growing in the steep terrain of the valley, andslow growth, so the need by Irpex lacteus liquid deep fermentation to obtain Irpexlacteus more.This paper adopts the water extraction of crude polysaccharide obtainedIrpex lacteus, in vivo experimental dialogue sac Irpex lacteus fr. via animal antifatigue and hypoxia tolerance of preliminary research activity, finally carried on theresearch and study of its stability of the quality standard of granule.According to single factor combined with response surface method onpolysaccharide capsule Irpex lacteus fr. extraction conditions were optimized,improve the content of polysaccharide.In the single factor experiment, the measuredwater extraction temperature80C, water extraction time3h, liquid water extractionratio of40:1, and through the response surface method to optimize the optimalextraction conditions of Irpex lacteus for extraction temperature86.72C, extractiontime3.66h, ratio of material to water43.89:1, regression test by Fang Chengyu get theoptimal water extraction results for10.62%.This thesis in autonomic activity test, can know Irpex lacteus autonomicactivities of mice and standing has no effect, so determine Irpex lacteus had noinfluence on the central nervous system.In water maze test, can know Irpex lacteusmemory and learning ability of mice had no significant influence. However, test, runIrpex lacteus in mice endurance rotarod test and weight loading swimming test, canknow Irpex lacteus had significant effect on mice physical strength and anti fatigue,and basically has dose dependence.Thus, through the behavior of simple test animal,basic can judge the basic activity of simple drugs. Irpex lacteus can maintain thebody’s energy, blocking the body lipid peroxidation, reducing MDA injury to the body;Irpex lacteus can also make the body in a state of hypoxia promotes the formation of antioxidant enzymes, increasing the activity of antioxidant enzymes, can acceleratethe removal of excess free radicals; Irpex lacteus promote adjustment role ofhypothalamic—pituitary—adrenal cortex, the CORT content and ACTH content inthe range to maintain relatively reasonable, make the body to hypoxia stimulation isrelatively slow down effect.This thesis is composed of the hypoxia tolerance test results can be seen, Irpexlacteus every dose group could prolong the survival time of mice. So judge Irpexlacteus may have an effect on hypoxia experiment. Followed by acute cerebralischemic test results, each group could Irpex lacteus prolonged in mice afterdecapitation opening times, once again illustrates the Irpex lacteus had effect onhypoxia experiment.Further by sodium nitrite poisoning test results show that, thegroup could Irpex lacteus prolonged the survival time of the mice, which illustratesIrpex lacteus has protective effect on hypoxia. Through maximum tolerance test onsac Irpex lacteus fr. extract safety evaluate. By the results showed, Irpex lacteusextract the maximum dosage is greater than5.0g/kg, in line with national safetyrequirements.This paper preliminarily established a quality standard of Irpex lacteus crudeextract granule, the granule is brown to dark brown, no bad smell, taste slightly sour,particle size uniformity, no obvious impurity, moisture content of not more than6%,are all melted, load difference in national standard within the microbial limit, meet thenational requirements, the content of polysaccharide was measured by phenol sulfuricacid method of not less than25mg/g; also accelerated stability test and long-termstability test to verify the stability of the preparation method of the good.