Migraine Model Rat Behavior Research Of Neurogenic Inflammation Induced By CGRP

Objective:Exogenous calcitonin gene related peptide(CGRP) was injected intorat dura mater, which would trigger neurogenic inflammation,mimic theactivation of the trigeminal vascular system in migraine attack.We hopedto establish a migraine model of rat dural neurogenic inflammationinduced by CGRP,observe the behavior of rats.Methods:60Clean level healthy male SD rats were randomly divided into6groups (n=10): group A (empty tube group)，group B (normal salinecontrol group), group C (CGRP1.5ug), group D (CGRP3ug), group E(CGRP6ug), group F (CGRP9ug).(1)Rats were fixed on the stereotaxic instrument,cathetered in parietal,restoring3to5days after surgery.(2)Rat dural mater was injected saline solution20ul or differentconcentrations of CGRP20ul through catheter in the parietal.(3)After the treatment in rat, we recorded the rat behavior performancefor four hours using the camera.Begin from the time of injecting,every30minutes as a period of time, observe and record the ratbahavior changes in each time period, including red ears, climbing cage,face-grooming,body-grooming,freezing behavior, restingbehavior, left hindpaw facial grooming.(4)The behavior results were analyzed by SPSS19.0statistical software.Results:(1)Rats in group A、group B and group C did not appear red ears, theother three groups of rats appeared red ears after1.5-2minutes. Thestarting time of red ear in group D、group E、group F is comparedbetween groups had no statistical significance (P>0.05), thedisappearing time and duration of red ear was statistically significant(P <0.05).(2)In0to30minutes period,the rat climbing cage behavior was comparedbetween group A and group B, there was no statistical significance (P>0.05),compared between group A and each drug group, eachdifference was statistically significant (P <0.05), but the differencebetween each drug group, there was no statistical significance (P>0.05). In30to240minutes period,differences between each twogroups, there were no statistical significance (P>0.05).(3)In0to30minutes period, rat face-grooming behavior was comparedbetween group A and group B, there was no statistical significance (P>0.05), compared between group A and group D、group E、group F,each difference was statistically significant (P <0.05), but thedifference between each drug group, there was no statistical significance (P>0.05). In30to240minutes period,differencesbetween each two groups, there were no statistical significance (P>0.05).(4)In0-240minutes period,rat body-grooming behavior was comparedbetween group A and group B, there was no statistical significance (P>0.05), compared between group A and each drug group, there wereno statistical significance (P>0.05),the difference between each twodrug group, there was no statistical significance (P>0.05).(5) In0-240minutes period,rat freezing behavior was compared betweengroup A and group B, there was no statistical significance (P>0.05),compared between group A and each drug group, there were nostatistical significance (P>0.05),the difference between each twodrug group, there was no statistical significance (P>0.05).(6) In0-240minutes period,rat resting behavior was compared betweengroup A and group B, there was no statistical significance (P>0.05),compared between group A and each drug group, there were nostatistical significance (P>0.05),the difference between each twodrug group, there was no statistical significance (P>0.05).(7)In0to30minutes period,rat immobile behavior was comparedbetween group A and group B, there was no statistical significance (P>0.05), group A was respectively compared with group D、group E、group F,each difference was statistically significant (P <0.05), the difference between each two drug group, there was no statisticalsignificance (P>0.05). In30to240minutes period,differencesbetween each two groups, there were no statistical significance (P>0.05).(8) In0-240minutes period,rat left hindpaw facial grooming behaviorwas compared between group A and group B, there was no statisticalsignificance (P>0.05), compared between group A and each druggroup, there were no statistical significance (P>0.05),the differencebetween each two drug group, there was no statistical significance (P>0.05).Conclusion:(1) Exogenous CGRP was injected into rat dura mater through catheter inthe parietal can successfully establish rat migraine model.(2)This rat migraine model showed the most obvious behaviorwithin the first30minutes.(3)6ug and9ug CGRP are better doses.(4) According to P15、P85standard, rats migraine model success rate ofgroup CGRP6ug and group CGRP9ug was90%.