Clinical Studies Of Amino Acid Metabolism In Patients With Colorectal Cancer

Objective：Metabolomics,as a new frontier subject following genomics, proteomics,transcriptomics, have a strong development momentum. Metabolomics reveals thenature of the metabolic activities of life sciences by measure and analysis metabolismgroup, using the method of NMR (nuclear magnetic resonance, NMR) technology, GC(gas chromatography, GC) techniques，liquid chromatography (liquid chromatogram,LC) techniques， capillary electrophoresis-mass spectrometry (capillaryelectrophoresismass spectrometry, CE-MS) technology, ultra-high performance liquidchromatography (ultra high performance liquid chromatograhy, UPLC) technology.Searching specific metabolites of tumor diseases by the dynamic body metabolicpathway, it is able to identify the metabolic differences of human disease states, andthen perform the classification and diagnosis of disease. In this study, armed at patientswith colorectal cancer in blood, urine specimens and paired with healthy blood andurine for the study, use of high performance liquid chromatography-massspectrometry (LC-MS/MS) detection and analysis, by comparion the differences in theexpression of amino acid metabolism in the two groups, we try to find the amino acidassociated with colorectal cancer, which is the potential biomarkers of screening. Inorder to, This study provides a new direction of searching meaningful biomarkers anddrug treatment to further reveal the pathogenesis and early diagnosis of colorectalcancer.Methods:, Morning fasting blood and urine samples were from25patients withcolorectal cancer,18healthy cases respectively. And samples were pretreated prior tothe application of high-performance liquid sample after treatment-mass spectrometryto analyze the profile of amino acid metabolism. Results: By analysis of amino acid metabolism changes in the serum and urine ofcolorectal cancer patients and paired healthy people using high performance liquid-mass spectrometry,16difference amino acid were initially screened.11kinds of aminoacid were statistically significant differences in the amino acid metabolite levels in theserum of Colorectal cancer patients and healthy control group (P <0.05). Where inarginine, histidine, glutamic acid, phenylalanine (P <0.05), and asparagine, lysine,tryptophan, valine, leucine, isoleucine, cysteine (P <0.01) differences between themetabolite is more significant.12kinds of amino acid differences were statisticallysignificant in amino acid metabolite levels in urine of Colorectal cancer patients andhealthy control group (P <0.05). Where in the phenylalanine (P <0.05), and arginine,histidine, asparagine, glutamine, valine, serine, leucine, isoleucine, proline, asparaginehistidine, glycine (P <0.01) difference between the metabolite is more significant.Seven differences amino acids were present both in the blood and urine specimens.Conclusion: There was a statistically significant increase in asparagine, cysteinemetabolite in the serum. And there was a significant decrease in lysine, tryptophan,valine, leucine, isoleucine significant metabolite in the serum. It showed significantupward trend in the urinary of asparagine, serine, glycine, histidine, isoleucine, leucinemetabolite. And there was a significantly decrease in arginine, glutamic appears,proline, aspartic acid metabolite in the urinary. such changes in amino acid metabolismare important in the early detection of colorectal cancer. Seven kinds of amino acids,which are arginine, asparagine, histidine, valine, phenylalanine, leucine, and isoleucine,are meaningful in both serum and urine samples. In these types of amino acids on thecorrelation with colorectal cancer, the seven kinds of amino acids, metabolitesappeared in both the serum and urine in the early diagnosis of metabolic products, aremore meaningful. These amino acids may be the important biomarkers in the screening,diagnosis and treatment of colorectal cancer.