The Protective Effects Of Ulinastatin In Rat Limb After Ischemia/Reperfusion Injury

Objective To investigate the protective effect and mechanism of ulinastatinin rat skeletal muscle ischemia-reperfusion injury.Methods72SPF grade rats were randomly divided into three groups:control group, ischemia-reperfusion group and ischemia-reperfusion-ulinastatingroup, n=24. Each group according to reperfusion time divided into0h,2h,4h,8h, at each time point six rats. Establishment of lower limb ischemia/reperfusionmodel. The same time, through the femoral vein blood collection equipmentseized; take skeletal muscle tissues were fixed, prepare electron microscopy, lightmicroscopy examination.They were measured in each group of animal skeletalmuscle in the event of reperfusion time points0h,2h,4h,8h plasma of LDH,CK content. Simultaneous detection of each group at different time pointshindlimb gastrocnemius tissue SOD and MDA content. Light microscopemorphology of skeletal muscle cells.Results Ischemic reperfusion plasma levels of LDH, CK content4hreached a peak, and then begin to decline, ischemia-reperfusion group andulinastatin group of LDH, CK level content at each time point was significantlyhigher (P<0.05), but ulinastatin group the different time points LDH, CK contentwas significantly lower than ischemia-reperfusion group (P<0.05). Thegastrocnemius organization ischemia-reperfusion group showed SOD activity waslower than the control group (P<0.05), which4h lowest; lipid peroxidationproduct MDA content were higher than that in the control group (P<0.05) and4hfor the highest; Ischemia-reperfusion group animal plasma SOD levels were lowerthan the control group (P<0.05);The ulinastatin group at each time pointgastrocnemius MDA content lower than the ischemia-reperfusion group (P<0.05),SOD content higher than the ischemia-reperfusion group.Conclusion1. Ulinastatin can reduce ischemia-reperfusion injury;2.Ulinastatin can effectively inhibit rat lower limb ischemia-reperfusion injury inskeletal muscle cell damage caused by the release of LDH and CK, so for large mouse limb ischemia-reperfusion injury of skeletal muscle has a protective effect;3. ischemia-reperfusion injury, the the ulinastatin group of SOD activity is higherthan the ischemia reperfusion group, which reflects oxygen MDA content of freeradicals on skeletal muscle damage than they ischemia reperfusion, the apparentprotective effect in scavenging oxygen free radicals and reduce hindlimbischemia-reperfusion injury.