Expression And Relation Of Galectin-3and MMP9in Gastric Cancer

1、BackgroundThe incidence rate of gastric cancer is high in the world. It is difficult to cure gastric cancer. The5-year survival rate is low. The recurrence and metastasis happen in most patients recently or in the future because the pathogenesis of molecular biology is complex and the pathological type is varied. And the patients would dead finally. It is beneficial to choose the good treatment and predict the progress of gastric cancer through researching the molecular mechanism of the occurrence and development in gastric cancer. The course of the occurrence、develop and metastasis is connection with inhibition of cell apoptosis、adhesion and degradation of cell-cell and between cell and matrix and formation of blood capillary. A lot of active molecule and signal transduction pathway is involved.Galectin-3belongs to chimera type. It has been detected in many kinds of cell such as enterocyte、brain cell、nephrocyte、bone cell、lung cancer cell. Matrix metalloproteinase-9(MMP-9) is one member of matrix metalloproteases(MMPS) family. MMP-9is a gelatinase. It can degrade collagen of the basement membrane. Galectin-3is one of substrates of MMP-9. The interaction of galectin-3and MMP-9can regulate the occurrence、development and invasion of tumours. Galectin-3and MMP-9is related to various cancers respectively. And they have close relation with gastric cancer. The combinational discussion of galectin-3and MMP-9about the expression and relation in gastric cancer is absent.Objects and methodsObjects46participants with gastric cancer and46health people with examination of gastroscopy in the Second Hospital of Jiaxing were collected from Jan.2011to Dec.2013. Gastric cancer tissue and precancerous tissue of gastric cancer patients and nomal gastric tisse of health people were choosed. The pathological diagnosis of all patients was clear.MethodsGastric cancer tissue and precancerous(including intestinal metaplasia and dysplasia) tissue of gastric cancer patients and nomal gastric tisse of health people were selected. The tissues were stained by hematoxylin-eosin staining. We bought rabbit anti-galectin-3polyclone antibody and rabbit anti-MMP-9monoclonal antibody. The expression of galectin-3and MMP9was investigated by SP immunohistochemistry.Judgment standard of immunohistochemistryThe judgment standard of immunohistochemistry for galectin-3and MMP-9was based on the colour and percent of the stained cells:the standard of colour:0-colourless,1-faint colour,2-medium colour,3-strong colour; positive cells count:1-1%to25%, 2-25%to50%,3-50%to75%,4-greater than75%; the positive judgment was in accordance with the product of the two scores(+-+++),3score was+,4score was++, greater than5score was+++.Statistical methodsThe SPSS19.0for Windows was used. The datas were analysed by;χ2-test, α=0.05. The consistency was analysed by Kappa test.ResultsThere were23patients with the serosal invasion、25patients with lymph nodes metastasis、41patients with low and middle differentiation、5patients with high differentiation. According to Lauren classification,35gastric cancers were intestinal-type carcinoma and11were mixed type. We found18cancers in Ⅰ stage,8cancers in Ⅱ stage,17cancers in Ⅲ stage and3cancers in Ⅳ stage by TNM stages.The expression of galectin-3The positive of galectin-3in gastric cancer、precancer and normal was73.913%、45.652%and8.696%. The positive expression was related with tumour invasion、lymph metastasis、pathological grade and TNM stage(P<0.05). It was non-related with Lauren classification (P>0.05).The expression of MMP-9The positive of MMP-9in gastric cancer、precancer and normal was69.565%、39.130%and6.522%. The positive expression was associated with tumour invasion、 lymph metastasis、pathological grade and TNM stage(P<0.05). It was non-related with Lauren classification (P>0.05). The relation of galectin-3and MMP-9The positive expression of galectin-3in gastric cancer had no relation to MMP-9.ConclusionThe expression of galectin-3and MMP9in gastric cancer and precancer was up-regulated. They were related with gastric cancer development and metastasis. Galectin-3and MMP-9would serve as a molecule markers.